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https://hdl.handle.net/2440/131959
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Type: | Journal article |
Title: | Genomic analysis of phylogenetic group B2 extraintestinal pathogenic E. coli causing infections in dogs in Australia |
Author: | Kidsley, A.K. O'Dea, M. Saputra, S. Jordan, D. Johnson, J.R. Gordon, D.M. Turni, C. Djordjevic, S.P. Abraham, S. Trott, D.J. |
Citation: | Veterinary Microbiology, 2020; 248:1-7 |
Publisher: | Elsevier |
Issue Date: | 2020 |
ISSN: | 0378-1135 1873-2542 |
Statement of Responsibility: | Amanda K. Kidsley, Mark O’Dea, Sugiyono Saputra, David Jordan, James R.Johnson, David M.Gordon |
Abstract: | This study investigated the prevalence of extraintestinal pathogenic E. coli (ExPEC)-associated sequence types (STs) from phylogenetic group B2 among 449 fluoroquinolone-susceptible dog clinical isolates from Australia. Isolates underwent PCR-based phylotyping and random amplified polymorphic DNA analysis to determine clonal relatedness. Of the 317 so-identified group B2 isolates, 77 underwent whole genome sequencing (WGS), whereas the remainder underwent PCR-based screening for ST complexes (STc) STc12, STc73, STc372, and ST131. The predominant ST was ST372 according to both WGS (31 % of 77) and ST-specific PCR (22 % of 240), followed by (per WGS) ST73 (17 %), ST12 (7 %), and ST80 (7 %). A WGS-based phylogenetic comparison of ST73 isolates from dogs, cats, and humans showed considerable overall phylogenetic diversity. Although most clusters were species-specific, some contained closely related human and animal (dog > cat) isolates. For dogs in Australia these findings both confirm ST372 as the predominant E. coli clonal lineage causing extraintestinal infections and clarify the importance of human-associated group B2 lineage ST73 as a cause of UTI, with some strains possibly being capable of bi-directional (i.e., dog-human and human-dog) transmission. |
Keywords: | Escherichia coli; companion animals; dogs; cats; genomics; virulence genes |
Rights: | © 2020 Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.vetmic.2020.108783 |
Grant ID: | http://purl.org/au-research/grants/arc/LP130100736 |
Published version: | http://dx.doi.org/10.1016/j.vetmic.2020.108783 |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest 8 |
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