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|Title:||Inertial microfluidic purification of CAR-T-Cell products|
|Citation:||Advanced Biology, 2022; 6(1):2101018-1-2101018-12|
|Mona T. Elsemary, Michelle F. Maritz, Louise E. Smith, Majid Warkiani, Veronika Bandara, Silvana Napoli, Simon C. Barry, Justin T. Coombs, and Benjamin Thier|
|Abstract:||Chimeric antigen receptor T (CAR-T) cell therapy is rapidly becoming a frontline cancer therapy. However, the manufacturing process is time-, labor- and cost-intensive, and it suffers from significant bottlenecks. Many CAR-T products fail to reach the viability release criteria set by regulators for commercial cell therapy products. This results in non-recoupable costs for the manufacturer and is detrimental to patients who may not receive their scheduled treatment or receive out-of-specification suboptimal formula-tion. It is demonstrated here that inertial microfluidics can, within minutes, efficiently deplete nonviable cells from low-viability CAR-T cell products. The percentage of viable cells increases from 40% (SD ± 0.12) to 71% (SD ± 0.09) for untransduced T cells and from 51% (SD ± 0.12) to 71% (SD ± 0.09) for CAR-T cells, which meets the clinical trials’ release parameters. In addition, the processing of CAR-T cells formulated in CryStor yields a 91% reduction in the amount of the cryoprotectant dimethyl sulfoxide. Inertial microfluidic processing has no detrimental effects on the proliferation and cytotoxicity of CAR-T cells. Interestingly, ≈50% of T-regulatory and T-suppressor cells are depleted, suggesting the potential for inertial microfluidic processing to tune the phenotypical composition of T-cell products.|
|Keywords:||CAR-T cell; cryoprotectant; manufacture; microfluidic; purification; viability|
|Description:||Published online: December 8, 2021|
|Rights:||© 2021 Wiley-VCH GmbH|
|Appears in Collections:||Paediatrics publications|
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