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Type: Journal article
Title: Exploring sensitive label-free multiplex analysis with Raman-coded microbeads and SERS-coded reporters
Author: Azhar, U.
Ahmed, Q.
Ishaq, S.
Alwahabi, Z.T.
Dai, S.
Citation: Biosensors, 2022; 12(2):121-1-121-13
Publisher: MDPI AG
Issue Date: 2022
ISSN: 2079-6374
Statement of
Umar Azhar, Qazi Ahmed, Saira Ishaq, Zeyad T. Alwahabi and Sheng Dai
Abstract: Suspension microsphere immunoassays are rapidly gaining attention in multiplex bioassays. Accurate detection of multiple analytes from a single measurement is critical in modern bioanalysis, which always requires complex encoding systems. In this study, a novel bioassay with Raman-coded antibody supports (polymer microbeads with different Raman signatures) and surface-enhanced Raman scattering (SERS)-coded nanotags (organic thiols on a gold nanoparticle surface with different SERS signatures) was developed as a model fluorescent, label-free, bead-based multiplex immunoassay system. The developed homogeneous immunoassays included two surface-functionalized monodisperse Raman-coded microbeads of polystyrene and poly(4-tert-butylstyrene) as the immune solid supports, and two epitope modified nanotags (self-assembled 4-mercaptobenzoic acid or 3-mercaptopropionic acid on gold nanoparticles) as the SERS-coded reporters. Such multiplex Raman/SERS-based microsphere immunoassays could selectively identify specific paratope-epitope interactions from one mixture sample solution under a single laser illumination, and thus hold great promise in future suspension multiplex analysis for diverse biomedical applications.
Keywords: SERS; Raman; microbeads; multiplex; immunoassays
Rights: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
DOI: 10.3390/bios12020121
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Appears in Collections:Chemical Engineering publications

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