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https://hdl.handle.net/2440/135300
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Type: | Journal article |
Title: | Antimicrobial susceptibility, plasmid replicon typing, phylogenetic grouping, and virulence potential of avian pathogenic and faecal Escherichia coli isolated from meat chickens in Australia |
Author: | Awawdeh, L. Turni, C. Mollinger, J.L. Henning, J. Cobbold, R.N. Trott, D.J. Gibson, J.S. Wakeman, D.L. |
Citation: | Avian Pathology, 2022; 51(4):349-360 |
Publisher: | Taylor & Francis |
Issue Date: | 2022 |
ISSN: | 0307-9457 1465-3338 |
Statement of Responsibility: | L. Awawdeha, C. Turnic, J. L. Mollingerd, J. Henninga, R. N. Cobbolda, D. J. Trotte, J. S. Gibsona and D. L. Wakeham |
Abstract: | Globally, avian colibacillosis is a leading cause of morbidity and mortality in poultry, associated with economic losses and welfare problems. Here, clinical avian pathogenic E. coli isolates (CEC; n=50) and faecal E. coli isolates from healthy (FEC; n=187) Australian meat chickens collected between 2006 and 2014 were subjected to antimicrobial susceptibility testing, phylogenetic grouping, plasmid replicon (PR) typing, multilocus sequence typing, and virulence gene (VG) profiling. Extended-spectrum cephalosporin (ESC)- and fluoroquinolone (FQ)-resistant E. coli isolates underwent further genetic characterisation. Significant proportions of CEC and FEC were respectively susceptible (13/50 [26%]; 48/187 [26%],) or MDR (9/50 [18%]; 26/187 [14%]) to 20 tested antimicrobials. Phylogenetic groups A and C, and PR types IncFIB and IncFrep were most commonly represented. Five tested CEC-associated VGs were more prevalent in CEC (≥90%) compared to FEC isolates (≤58%). Some isolates (CEC n=3; FEC n=7) were resistant to ESCs and/or FQs and possessed signature mutations in chromosomal FQ target genes and plasmid-mediated qnrS, blaCMY-2, and blaDHA-1 genes. Sequence type 354 (n=4), associated with extraintestinal infections in a broad range of hosts, was prevalent among the ESC- and/or FQ-resistant FEC.This study confirmed the existence of a small reservoir of ESC- and FQ-resistant E. coli in Australian commercial meat chickens despite the absence of use in the industry of these drug classes. Otherwise, a diversity of VGs and PR types in both faecal and clinical E. coli populations were identified. It's hypothesised that the source of ESC- and FQ-resistant E. coli may be external to poultry production facilities.Highlights1. Low-level resistance to older and newer generation antimicrobial drugs detected2. The most common sequence type (ST) associated with FQ resistance was ST354 (4/10)3. A small proportion of CEC (n=3) and FEC (n=7) were resistant to ESCs and/or FQs. |
Keywords: | avian colibacillosis Antimicrobial resistance Avian pathogenic Escherichia coli Extended-spectrum cephalosporins Fluoroquinolones Phylogenetic groups Plasmid replicon virulence gene |
Description: | Published online: 20 May 2022 |
Rights: | © 2022 Houghton Trust Ltd |
DOI: | 10.1080/03079457.2022.2065969 |
Grant ID: | http://purl.org/au-research/grants/arc/LP130100736 |
Published version: | http://dx.doi.org/10.1080/03079457.2022.2065969 |
Appears in Collections: | Animal and Veterinary Sciences publications |
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