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https://hdl.handle.net/2440/139571
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Type: | Journal article |
Title: | Enhanced Bioactivity of Enzyme/MOF Biocomposite via Host Framework Engineering |
Author: | Liang, W. Flint, K. Yao, Y. Wu, J. Wang, L. Doonan, C. Huang, J. |
Citation: | Journal of the American Chemical Society, 2023; 145(37):20365-20374 |
Publisher: | AMER CHEMICAL SOC |
Issue Date: | 2023 |
ISSN: | 0002-7863 1520-5126 |
Statement of Responsibility: | Weibin Liang, Kate Flint, Yuchen Yao, Jiacheng Wu, Lizhuo Wang, Christian Doonan, and Jun Huang |
Abstract: | This study reports the successful development of a sustainable synthesis protocol for a phase-pure metal azolate framework (MAF-6) and its application in enzyme immobilization. An esterase@MAF-6 biocomposite was synthesized, and its catalytic performance was compared with that of esterase@ZIF-8 and esterase@ZIF-90 in transesterification reactions. Esterase@MAF-6, with its large pore aperture, showed superior enzymatic performance compared to esterase@ZIF-8 and esterase@ZIF-90 in catalyzing transesterification reactions using both n-propanol and benzyl alcohol as reactants. The hydrophobic nature of the MAF-6 platform was shown to activate the immobilized esterase into its open-lid conformation, which exhibited a 1.5- and 4-times enzymatic activity as compared to free esterase in catalyzing transesterification reaction using n-propanol and benzyl alcohol, respectively. The present work offers insights into the potential of MAF-6 as a promising matrix for enzyme immobilization and highlights the need to explore MOF matrices with expanded pore apertures to broaden their practical applications in biocatalysis. |
Keywords: | Biopolymers; encapsulation; metal organic frameworks; peptides and proteins; transesterification |
Rights: | © 2023 American Chemical Society |
DOI: | 10.1021/jacs.3c05488 |
Grant ID: | http://purl.org/au-research/grants/arc/DE220100583 http://purl.org/au-research/grants/arc/DP220102851 |
Published version: | http://dx.doi.org/10.1021/jacs.3c05488 |
Appears in Collections: | Chemistry and Physics publications |
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