Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/14463
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorColler, J.en
dc.contributor.authorSomogyi, A.en
dc.contributor.authorBochner, F.en
dc.date.issued1998en
dc.identifier.citationJournal of Chromatography B.., 1998; 719(1-2):87-92en
dc.identifier.issn0378-4347en
dc.identifier.issn1878-5603en
dc.identifier.urihttp://hdl.handle.net/2440/14463-
dc.description.abstractA high-performance liquid chromatographic assay for the quantification of the oxidative metabolites of flunitrazepam, 3-hydroxyflunitrazepam and desmethylflunitrazepam, in human liver microsomal incubations was developed. Both metabolites were quantifiable in a single assay following a solvent extraction and reversed-phase high-performance liquid chromatography with UV detection. Standard curve concentrations for both metabolites ranged from 0.2 to 10 microM. Assay performance was determined using quality control samples and the intra- and inter-day accuracy and precision as determined by the coefficient of variations which were less than 15% (0.5-6 microM) for both metabolites. This method provides good precision and accuracy for use in kinetic studies of the oxidative metabolism of flunitrazepam in human liver microsomes.en
dc.language.isoenen
dc.publisherELSEVIER SCIENCE BVen
dc.subjectMicrosomes, Liver; Humans; Flunitrazepam; Anti-Anxiety Agents; Calibration; Chromatography, High Pressure Liquid; Spectrophotometry, Ultraviolet; Reproducibility of Results; Reference Standardsen
dc.titleQuantification of flunitrazepam's oxidative metabolites, 3-hydroxyflunitrazepam and desmethylflunitrazepam, in hepatic microsomal incubations by high performance liquid chromatography.en
dc.typeJournal articleen
dc.identifier.rmid0030003398en
dc.identifier.doi10.1016/S0378-4347(98)00383-1en
dc.identifier.pubid67404-
pubs.library.collectionPharmacology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidColler, J. [0000-0002-8273-5048]en
dc.identifier.orcidSomogyi, A. [0000-0003-4779-0380]en
Appears in Collections:Pharmacology publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.