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Type: Journal article
Title: Effects of Ca2+ concentration on GABAB receptor function in rat neocortical slices
Author: Ong, J.
Kerr, D.
Citation: Canadian Journal of Physiology and Pharmacology, 2000; 78(6):507-511
Publisher: Natl Research Council Canada
Issue Date: 2000
ISSN: 0008-4212
Abstract: In rat neocortical slices maintained in Mg2+-free Kreb's medium, the effects of Ca2+ concentration on repetitive spontaneous discharges and on GABA(B) receptor-mediated responses were investigated. Over a concentration range of 0.3-2.4 mM Ca2+, there was a reduction of discharge amplitude, with a 50 +/- 6.5% reduction in 0.3 mM Ca2+, whilst the burst frequency remained unaffected. Baclofen, the GABA(B) receptor agonist, produced a concentration-dependent depression of discharge frequency, reversibly antagonised by the antagonist (+)-(S)-5,5-dimethylmorpholinyl-2-acetic acid (Sch 50911). The EC50 value for baclofen in 2.4 mM Ca2+ was 11 microM, while the EC50 values in 0.3, 0.6, 1.2, and 1.8 mM Ca2+ were 1.3, 2.5, 3.6, and 10 microM, respectively, resulting in 8.5, 4.4, 3.1, and 1.1-fold leftward shifts. This enhanced action of baclofen in low extracellular Ca2+ concentrations in the neocortex may be the result of a lower concentration gradient which reinforces the action of baclofen.
Keywords: Neocortex; Animals; Rats; Rats, Sprague-Dawley; Calcium Chloride; Baclofen; Receptors, GABA-B; GABA Agonists; Synaptic Transmission; Male
RMID: 0001000827
DOI: 10.1139/y00-017
Appears in Collections:Anaesthesia and Intensive Care publications

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