Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/17812
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | The rothein peptides from the skin secretion of Roth's tree frog Litoria rothii. Sequence determination using positive and negative ion electrospray mass spectrometry |
Author: | Brinkworth, C. Bowie, J. Bilusich, D. Tyler, M. |
Citation: | Rapid Communications in Mass Spectrometry, 2005; 19(18):2716-2724 |
Publisher: | John Wiley & Sons Ltd |
Issue Date: | 2005 |
ISSN: | 0951-4198 1097-0231 |
Statement of Responsibility: | Craig S. Brinkworth, John H. Bowie, Daniel Bilusich, Michael J. Tyler |
Abstract: | The secretion from the dorsal glands of the frog Litoria rothii contains a series of new peptides including rothein 1 (SVSNIPESIGF-OH, a neuropeptide which contracts smooth muscle), a number of inactive rothein 2 and 3 peptides (e.g. rothein 2.1, AGGLDDLLEPVLNSADNLVHGL-OH), and a new proline rich peptide, named rothein 4.1 (AEILFGDVRPPWMPPPIFPEMP-OH), which shows neither antimicrobial nor neuronal nitric oxide synthase (nNOS) activity. Two known neuropeptides of the caerulein family [e.g. caerulein, pEQDY(SO3)TGWMDF-NH2] together with a series of known caerin 1 antibiotic and nNOS-inhibiting peptides (e.g. caerin 1.1, GLLSVLGSVAKHVLPHVVPVIAEHL-NH2) were also identified. Positive ion electrospray mass spectrometry (ES-MS) was used as the primary method to investigate the sequences of the new peptides. Negative ion ES-MS was used to fill in any gaps in the positive ion data and, finally, Edman automated sequencing was used to differentiate between Leu and Ile and to confirm the sequences determined by mass spectrometry. |
Keywords: | Skin Animals Ranidae Peptides Spectrometry, Mass, Electrospray Ionization Sequence Analysis, Protein Amino Acid Sequence Molecular Sequence Data |
Description: | The definitive version may be found at www.wiley.com |
DOI: | 10.1002/rcm.2098 |
Published version: | http://dx.doi.org/10.1002/rcm.2098 |
Appears in Collections: | Aurora harvest 6 Chemistry publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.