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https://hdl.handle.net/2440/1883
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Type: | Journal article |
Title: | Simultaneous fixation using glutaraldehyde and osmium tetroxide or potassium ferricyanide-reduced osmium for the preservation of monogenean flatworms: An assessment for Merizocotyle icopae |
Author: | Cribb, B. Armstrong, W. Whittington, I. |
Citation: | Microscopy Research and Technique, 2004; 63(2):102-110 |
Publisher: | Wiley-Liss |
Issue Date: | 2004 |
ISSN: | 1059-910X 1097-0029 |
Statement of Responsibility: | Bronwen Cribb, Wendy Armstrong, Ian Whittington |
Abstract: | <jats:title>Abstract</jats:title><jats:p>Simultaneous fixation was investigated for a marine organism: the monogenean flatworm ectoparasite <jats:italic>Merizocotyle icopae</jats:italic>. Four protocols for primary fixation were compared: 3% glutaraldehyde alone in 0.1M cacodylate buffer for a minimum of 2 hours; 1% glutaraldehyde in combination with 1% osmium tetroxide, both in 0.1M cacodylate buffer, until tissues darkened (5–20 minutes); 1% glutaraldehyde in 0.1M cacodylate buffer in combination with 0.5% potassium ferricyanide‐reduced osmium until tissues darkened (5–20 minutes); 1% glutaraldehyde in combination with 1% osmium tetroxide, both in 0.1M cacodylate buffer, for 30 minutes. The study confirms that the standard method for transmission electron microscopic fixation (first listed protocol) routinely applied to platyhelminths is optimal for ultrastructural preservation, but some simultaneous fixation methods (second and third listed protocols) are acceptable when rapid immobilization is needed. Scanning electron microscopic preparations may be improved using simultaneous primary fixation. Microsc. Res. Tech. 63:102–110, 2004. © 2004 Wiley‐Liss, Inc.</jats:p> |
Keywords: | Mixed fixative parasite platyhelminths electron microscopy SEM TEM |
Description: | Published in Microscopy Research and Technique, 2004; 63 (2):102-110 at www.interscience.wiley.com |
DOI: | 10.1002/jemt.20015 |
Published version: | http://www3.interscience.wiley.com/cgi-bin/abstract/106577093/ABSTRACT |
Appears in Collections: | Aurora harvest 6 Earth and Environmental Sciences publications Environment Institute publications |
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