Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/23097
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Use of ethylenediaminetetraacetic acid for in vivo stripping of columnar mucosa: Pilot study in an experimental model
Author: France, M.
Drew, P.
Ruszkiewicz, A.
Jamieson, G.
Watson, D.
Citation: Australian and New Zealand Journal of Surgery, 2006; 76(5):392-397
Publisher: Blackwell Science Asia
Issue Date: 2006
ISSN: 1445-1433
1445-2197
Statement of
Responsibility: 
Michael France, Paul A. Drew, Andrew Ruszkiewicz, Glyn G. Jamieson and David I. Watson
Abstract: Background: Barrett’s oesophagus is an important clinical problem that can lead to oesophageal adenocarcinoma. A variety of mucosal ablation strategies are now being applied in an attempt to prevent this, although they all can cause complications. Animal experiments that suggest that ethylenediaminetetraacetic acid (EDTA) could be a novel agent for ablation of Barrett’s oesophagus are reported in this article. Methods: Dark Agouti rats were used in all experiments. To determine the feasibility of using EDTA to strip intestinal type mucosa, segments of the small intestine were exposed in vitro to EDTA at various concentrations with or without agitation. The conditions required for EDTA to strip mucosa from a vascularized loop of small bowel were then optimized in vivo. Cannulated small bowel loops were irrigated with different concentrations of EDTA with or without pulsation of the irrigation solution. The effect of similar treatments on the normal mucosa of the rat oesophagus was then determined. Mucosal healing after EDTA stripping was studied in an isolated small bowel loop survival model. Results: Ethylenediaminetetraacetic acid with agitation or pulsation resulted in stripping of the intestinal columnar mucosa in vitro and in vivo. The extent was influenced by the concentration of EDTA and duration of exposure. Squamous epithelium was relatively resistant to stripping. In the survival model the small bowel mucosa regenerated without stricture formation. Conclusion: Small bowel columnar mucosa can be removed by EDTA in vivo without stricture formation. A refinement of this approach could be applicable to the ablation of Barrett’s oesophagus.
Keywords: Barrett's oesophagus; In vivo; Mucosa; Small bowel
RMID: 0020060631
DOI: 10.1111/j.1445-2197.2006.03729.x
Appears in Collections:Pathology publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.