Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/23213
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Type: Journal article
Title: ICAM-1 expression in adipose tissue: effects of diet-induced obesity in mice
Author: Brake, D.
O'Brian Smith, E.
Mersmann, H.
Smith, C.
Robker, R.
Citation: American Journal of Physiology-Cell Physiology, 2006; 291(6):C1232-C1239
Publisher: Amer Physiological Soc
Issue Date: 2006
ISSN: 0363-6143
1522-1563
Statement of
Responsibility: 
Danett K. Brake, E. O'Brian Smith, Harry Mersmann, C. Wayne Smith and Rebecca L. Robker
Abstract: Obesity has been linked to cardiovascular disease, hypertension, diabetes and the metabolic syndrome, with elevated markers of systemic inflammation. Intercellular adhesion molecule-1 (ICAM-1) is a transmembrane adhesion molecule involved in leukocyte migration to sites of inflammation. In human obesity, elevated expression of the soluble form of ICAM-1 (sICAM-1) is positively correlated with abdominal fat deposition. Increases in adiposity have also been correlated with macrophage infiltration into adipose tissue. Here we investigate adipose tissue production and transcriptional regulation of ICAM-1 in a mouse model of dietary obesity. After feeding mice a high-fat diet, ICAM-1 expression in serum and adipose tissue was analyzed by ELISA, Northern blotting, real-time quantitative PCR, and flow cytometry. After 6 mo on the high-fat diet, sICAM-1 levels significantly correlated with body weight and abdominal fat mass. ICAM-1 mRNA was expressed in adipose tissue of mice, with significantly higher levels in males than females. After only 3 wk, there were adipose tissue-specific increases in mRNAs for ICAM-1, IL-6, and monocyte chemoattractant protein-1 (MCP-1) in male mice. Analysis of the stromal-vascular fraction of male adipose tissue revealed CD11b-negative cells with increased surface ICAM-1 and CD34. We also found two populations of F4/80+, CD11b+, ICAM-1+ cells, one of which also expressed CD14 and CD11c and was increased in response to a high-fat diet. These results indicate that within 3 wk on a high-fat diet, male mice exhibited significant increases in pro-inflammatory factors and immune cell infiltration in adipose tissue that may represent links between obesity and its associated inflammatory complications.
Keywords: intercellular-adhesion molecule-1; monocyte chemoattractant protein-1; necrosis-factor-alpha; insulin-resistance; dendritic cells; cardiac myocytes; inflammation; adipocytes; adherence; cleavage
Rights: Copyright © 2006 by the American Physiological Society.
RMID: 0020061703
DOI: 10.1152/ajpcell.00008.2006
Published version: http://ajpcell.physiology.org/cgi/content/short/291/6/C1232
Appears in Collections:Obstetrics and Gynaecology publications

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