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|Title:||ICAM-1 expression in adipose tissue: effects of diet-induced obesity in mice|
O'Brian Smith, E.
|Citation:||American Journal of Physiology-Cell Physiology, 2006; 291(6):C1232-C1239|
|Publisher:||Amer Physiological Soc|
|Danett K. Brake, E. O'Brian Smith, Harry Mersmann, C. Wayne Smith and Rebecca L. Robker|
|Abstract:||Obesity has been linked to cardiovascular disease, hypertension, diabetes and the metabolic syndrome, with elevated markers of systemic inflammation. Intercellular adhesion molecule-1 (ICAM-1) is a transmembrane adhesion molecule involved in leukocyte migration to sites of inflammation. In human obesity, elevated expression of the soluble form of ICAM-1 (sICAM-1) is positively correlated with abdominal fat deposition. Increases in adiposity have also been correlated with macrophage infiltration into adipose tissue. Here we investigate adipose tissue production and transcriptional regulation of ICAM-1 in a mouse model of dietary obesity. After feeding mice a high-fat diet, ICAM-1 expression in serum and adipose tissue was analyzed by ELISA, Northern blotting, real-time quantitative PCR, and flow cytometry. After 6 mo on the high-fat diet, sICAM-1 levels significantly correlated with body weight and abdominal fat mass. ICAM-1 mRNA was expressed in adipose tissue of mice, with significantly higher levels in males than females. After only 3 wk, there were adipose tissue-specific increases in mRNAs for ICAM-1, IL-6, and monocyte chemoattractant protein-1 (MCP-1) in male mice. Analysis of the stromal-vascular fraction of male adipose tissue revealed CD11b-negative cells with increased surface ICAM-1 and CD34. We also found two populations of F4/80+, CD11b+, ICAM-1+ cells, one of which also expressed CD14 and CD11c and was increased in response to a high-fat diet. These results indicate that within 3 wk on a high-fat diet, male mice exhibited significant increases in pro-inflammatory factors and immune cell infiltration in adipose tissue that may represent links between obesity and its associated inflammatory complications.|
|Keywords:||intercellular-adhesion molecule-1; monocyte chemoattractant protein-1; necrosis-factor-alpha; insulin-resistance; dendritic cells; cardiac myocytes; inflammation; adipocytes; adherence; cleavage|
|Rights:||Copyright © 2006 by the American Physiological Society.|
|Appears in Collections:||Obstetrics and Gynaecology publications|
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