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https://hdl.handle.net/2440/23213
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dc.contributor.author | Brake, D. | - |
dc.contributor.author | O'Brian Smith, E. | - |
dc.contributor.author | Mersmann, H. | - |
dc.contributor.author | Smith, C. | - |
dc.contributor.author | Robker, R. | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | American Journal of Physiology: Cell Physiology, 2006; 291(6):C1232-C1239 | - |
dc.identifier.issn | 0363-6143 | - |
dc.identifier.issn | 1522-1563 | - |
dc.identifier.uri | http://hdl.handle.net/2440/23213 | - |
dc.description.abstract | Obesity has been linked to cardiovascular disease, hypertension, diabetes and the metabolic syndrome, with elevated markers of systemic inflammation. Intercellular adhesion molecule-1 (ICAM-1) is a transmembrane adhesion molecule involved in leukocyte migration to sites of inflammation. In human obesity, elevated expression of the soluble form of ICAM-1 (sICAM-1) is positively correlated with abdominal fat deposition. Increases in adiposity have also been correlated with macrophage infiltration into adipose tissue. Here we investigate adipose tissue production and transcriptional regulation of ICAM-1 in a mouse model of dietary obesity. After feeding mice a high-fat diet, ICAM-1 expression in serum and adipose tissue was analyzed by ELISA, Northern blotting, real-time quantitative PCR, and flow cytometry. After 6 mo on the high-fat diet, sICAM-1 levels significantly correlated with body weight and abdominal fat mass. ICAM-1 mRNA was expressed in adipose tissue of mice, with significantly higher levels in males than females. After only 3 wk, there were adipose tissue-specific increases in mRNAs for ICAM-1, IL-6, and monocyte chemoattractant protein-1 (MCP-1) in male mice. Analysis of the stromal-vascular fraction of male adipose tissue revealed CD11b-negative cells with increased surface ICAM-1 and CD34. We also found two populations of F4/80+, CD11b+, ICAM-1+ cells, one of which also expressed CD14 and CD11c and was increased in response to a high-fat diet. These results indicate that within 3 wk on a high-fat diet, male mice exhibited significant increases in pro-inflammatory factors and immune cell infiltration in adipose tissue that may represent links between obesity and its associated inflammatory complications. | - |
dc.description.statementofresponsibility | Danett K. Brake, E. O'Brian Smith, Harry Mersmann, C. Wayne Smith and Rebecca L. Robker | - |
dc.language.iso | en | - |
dc.publisher | Amer Physiological Soc | - |
dc.rights | Copyright © 2006 by the American Physiological Society. | - |
dc.source.uri | http://ajpcell.physiology.org/cgi/content/short/291/6/C1232 | - |
dc.subject | intercellular-adhesion molecule-1 | - |
dc.subject | monocyte chemoattractant protein-1 | - |
dc.subject | necrosis-factor-alpha | - |
dc.subject | insulin-resistance | - |
dc.subject | dendritic cells | - |
dc.subject | cardiac myocytes | - |
dc.subject | inflammation | - |
dc.subject | adipocytes | - |
dc.subject | adherence | - |
dc.subject | cleavage | - |
dc.title | ICAM-1 expression in adipose tissue: effects of diet-induced obesity in mice | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1152/ajpcell.00008.2006 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Robker, R. [0000-0002-1538-4604] | - |
Appears in Collections: | Aurora harvest 2 Obstetrics and Gynaecology publications |
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