Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/23994
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Type: Journal article
Title: Reduced virulence of an fliC mutant of Shiga-Toxigenic Escherichia coli O113 : H21
Author: Rogers, T.
Paton, J.
Wang, H.
Talbot, U.
Paton, A.
Citation: Infection and Immunity, 2006; 74(3):1962-1966
Publisher: Amer Soc Microbiology
Issue Date: 2006
ISSN: 0019-9567
1098-5522
Statement of
Responsibility: 
Trisha J. Rogers, James C. Paton, Hui Wang, Ursula M. Talbot, and Adrienne W. Paton
Abstract: The contribution of flagellin to the virulence of the O113:H21 Shiga-toxigenic Escherichia coli (STEC) strain 98NK2 was investigated in the streptomycin-treated mouse model. Groups of mice were challenged with either the wild-type STEC or a fliC deletion derivative thereof. There was no difference in the level of gut colonization by the two strains, but the fliC mutant was significantly less virulent than its parent; the overall survival rates were 43.7% and 81.2%, respectively (P < 0.025). This is the first report of a nontoxic accessory virulence factor contributing to a fatal outcome of STEC infection in this model. Although H21 FliC is known to be a potent inducer of CXC chemokines, including interleukin 8, there was no obvious difference in the recruitment of polymorphonuclear leukocytes to the intestinal epithelium of mice challenged with either strain. However, immunofluorescence microscopy suggested that the fliC mutant was less capable of forming a close association with the colonic epithelium. This may have reduced the uptake of Stx2 by mice infected with the mutant.
Keywords: Animals; Mice; Escherichia coli; Escherichia coli Infections; Streptomycin; Flagellin; Shiga Toxins; Virulence; Mutation
Description: Copyright © 2006, American Society for Microbiology
RMID: 0020060268
DOI: 10.1128/IAI.74.3.1962-1966.2006
Published version: http://iai.asm.org/cgi/content/abstract/74/3/1962
Appears in Collections:Molecular and Biomedical Science publications

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