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https://hdl.handle.net/2440/27516
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Type: | Journal article |
Title: | Multiplex PCR for direct detection of Shiga toxigenic Escherichia coli strains producing the novel subtilase cytotoxin |
Author: | Paton, A. Paton, J. |
Citation: | Journal of Clinical Microbiology, 2005; 43(6):2944-2947 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2005 |
ISSN: | 0095-1137 1098-660X |
Statement of Responsibility: | Adrienne W. Paton and James C. Paton |
Abstract: | We have recently described a novel AB5 subtilase cytotoxin produced by certain Shiga toxigenic Escherichia coli (STEC) strains. This potentially lethal toxin may contribute to severe gastrointestinal and systemic disease in humans. In this study we have developed a trivalent PCR assay for the detection of the novel toxin A subunit gene subA, as well as stx1 and stx2. The three primer pairs used in the assay do not interfere with each other and generate amplification products of 556, 180, and 255 bp, respectively. The assay can be used for determining the toxin genotype of STEC isolates, as well as for direct detection of toxin genes in primary fecal culture extracts. |
Keywords: | Feces Humans Escherichia coli Escherichia coli Infections Diarrhea Serine Endopeptidases Escherichia coli Proteins Protein Subunits DNA, Bacterial Shiga Toxins Cytotoxins Polymerase Chain Reaction Shiga Toxin 1 Shiga Toxin 2 |
Description: | Copyright © 2005, American Society for Microbiology. All Rights Reserved. |
DOI: | 10.1128/JCM.43.6.2944-2947.2005 |
Published version: | http://jcm.asm.org/cgi/content/abstract/43/6/2944 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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