Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Multiplex PCR for direct detection of Shiga toxigenic Escherichia coli strains producing the novel subtilase cytotoxin|
|Citation:||Journal of Clinical Microbiology, 2005; 43(6):2944-2947|
|Publisher:||Amer Soc Microbiology|
|Adrienne W. Paton and James C. Paton|
|Abstract:||We have recently described a novel AB5 subtilase cytotoxin produced by certain Shiga toxigenic Escherichia coli (STEC) strains. This potentially lethal toxin may contribute to severe gastrointestinal and systemic disease in humans. In this study we have developed a trivalent PCR assay for the detection of the novel toxin A subunit gene subA, as well as stx1 and stx2. The three primer pairs used in the assay do not interfere with each other and generate amplification products of 556, 180, and 255 bp, respectively. The assay can be used for determining the toxin genotype of STEC isolates, as well as for direct detection of toxin genes in primary fecal culture extracts.|
|Keywords:||Feces; Humans; Escherichia coli; Escherichia coli Infections; Diarrhea; Serine Endopeptidases; Escherichia coli Proteins; Protein Subunits; DNA, Bacterial; Shiga Toxins; Cytotoxins; Polymerase Chain Reaction; Shiga Toxin 1; Shiga Toxin 2|
|Description:||Copyright © 2005, American Society for Microbiology. All Rights Reserved.|
|Appears in Collections:||Molecular and Biomedical Science publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.