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Type: Journal article
Title: Multiplex PCR for direct detection of Shiga toxigenic Escherichia coli strains producing the novel subtilase cytotoxin
Author: Paton, A.
Paton, J.
Citation: Journal of Clinical Microbiology, 2005; 43(6):2944-2947
Publisher: Amer Soc Microbiology
Issue Date: 2005
ISSN: 0095-1137
Statement of
Adrienne W. Paton and James C. Paton
Abstract: We have recently described a novel AB5 subtilase cytotoxin produced by certain Shiga toxigenic Escherichia coli (STEC) strains. This potentially lethal toxin may contribute to severe gastrointestinal and systemic disease in humans. In this study we have developed a trivalent PCR assay for the detection of the novel toxin A subunit gene subA, as well as stx1 and stx2. The three primer pairs used in the assay do not interfere with each other and generate amplification products of 556, 180, and 255 bp, respectively. The assay can be used for determining the toxin genotype of STEC isolates, as well as for direct detection of toxin genes in primary fecal culture extracts.
Keywords: Feces; Humans; Escherichia coli; Escherichia coli Infections; Diarrhea; Serine Endopeptidases; Escherichia coli Proteins; Protein Subunits; DNA, Bacterial; Shiga Toxins; Cytotoxins; Polymerase Chain Reaction; Shiga Toxin 1; Shiga Toxin 2
Description: Copyright © 2005, American Society for Microbiology. All Rights Reserved.
RMID: 0020050537
DOI: 10.1128/JCM.43.6.2944-2947.2005
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Appears in Collections:Molecular and Biomedical Science publications

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