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Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/27516
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dc.contributor.authorPaton, A.-
dc.contributor.authorPaton, J.-
dc.date.issued2005-
dc.identifier.citationJournal of Clinical Microbiology, 2005; 43(6):2944-2947-
dc.identifier.issn0095-1137-
dc.identifier.issn1098-660X-
dc.identifier.urihttp://hdl.handle.net/2440/27516-
dc.descriptionCopyright © 2005, American Society for Microbiology. All Rights Reserved.-
dc.description.abstractWe have recently described a novel AB5 subtilase cytotoxin produced by certain Shiga toxigenic Escherichia coli (STEC) strains. This potentially lethal toxin may contribute to severe gastrointestinal and systemic disease in humans. In this study we have developed a trivalent PCR assay for the detection of the novel toxin A subunit gene subA, as well as stx1 and stx2. The three primer pairs used in the assay do not interfere with each other and generate amplification products of 556, 180, and 255 bp, respectively. The assay can be used for determining the toxin genotype of STEC isolates, as well as for direct detection of toxin genes in primary fecal culture extracts.-
dc.description.statementofresponsibilityAdrienne W. Paton and James C. Paton-
dc.language.isoen-
dc.publisherAmer Soc Microbiology-
dc.source.urihttp://jcm.asm.org/cgi/content/abstract/43/6/2944-
dc.subjectFeces-
dc.subjectHumans-
dc.subjectEscherichia coli-
dc.subjectEscherichia coli Infections-
dc.subjectDiarrhea-
dc.subjectSerine Endopeptidases-
dc.subjectEscherichia coli Proteins-
dc.subjectProtein Subunits-
dc.subjectDNA, Bacterial-
dc.subjectShiga Toxins-
dc.subjectCytotoxins-
dc.subjectPolymerase Chain Reaction-
dc.subjectShiga Toxin 1-
dc.subjectShiga Toxin 2-
dc.titleMultiplex PCR for direct detection of Shiga toxigenic Escherichia coli strains producing the novel subtilase cytotoxin-
dc.typeJournal article-
dc.identifier.doi10.1128/JCM.43.6.2944-2947.2005-
pubs.publication-statusPublished-
dc.identifier.orcidPaton, J. [0000-0001-9807-5278]-
Appears in Collections:Aurora harvest 6
Molecular and Biomedical Science publications

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