Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/27526
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Type: Journal article
Title: Protective immunization of mice with an active-site mutant of subtilase cytotoxin of Shiga toxin-producing Escherichia coli
Author: Talbot, U.
Paton, J.
Paton, A.
Citation: Infection and Immunity, 2005; 73(7):4432-4436
Publisher: Amer Soc Microbiology
Issue Date: 2005
ISSN: 0019-9567
1098-5522
Statement of
Responsibility: 
Ursula M. Talbot, James C. Paton, and Adrienne W. Paton
Abstract: We have recently described a novel AB5 cytotoxin produced by certain Shiga toxin-producing Escherichia coli strains. The A subunit of this toxin is a subtilase-like serine protease, while the B pentamer mediates binding to host cell glycolipid receptors. The subtilase cytotoxin is lethal for mice, causing extensive microvascular thrombosis as well as necrosis in the brain, kidneys, and liver. In the present study, we have immunized mice with a purified derivative of the toxin with a Ser272 Ala mutation in the A subunit which abolishes cytotoxicity. This elicited strong antibody responses, as judged by enzyme-linked immunosorbent assay, which conferred protection against intraperitoneal challenge with purified toxin. Immunized mice were also protected from weight loss resulting from oral challenge with an E. coli K-12 clone expressing the active toxin.
Keywords: Animals; Mice, Inbred BALB C; Mice; Escherichia coli; Serine Endopeptidases; Protein Subunits; Shiga Toxin; Cytotoxins; Escherichia coli Vaccines; Antibodies, Bacterial; Enzyme-Linked Immunosorbent Assay; Immunization; Binding Sites; Mutation; Male
RMID: 0020050704
DOI: 10.1128/IAI.73.7.4432-4436.2005
Published version: http://iai.asm.org/cgi/content/abstract/73/7/4432
Appears in Collections:Molecular and Biomedical Science publications

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