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https://hdl.handle.net/2440/27703
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Type: | Journal article |
Title: | The molecular regulation of stilbene phytoalexin biosynthesis in Vitis vinifera during grape berry development |
Author: | Bais, Anthony J. Murphy, Peter J. Dry, Ian B. |
Citation: | Australian Journal of Plant Physiology, 2000; 27(5):425-433 |
Publisher: | CSIRO Publishing |
Issue Date: | 2000 |
ISSN: | 0310-7841 |
School/Discipline: | School of Agriculture, Food and Wine : Plant and Food Science |
Statement of Responsibility: | Anthony J. Bais, Peter J. Murphy and Ian B. Dry |
Abstract: | The molecular regulation of stilbene phytoalexin biosynthesis in developing Vitis vinifera L. grape berries was investigated using a UV induction system. Berries were collected at 1, 5, 10 and 16 weeks post-flowering from the cultivars Shiraz, Semillon, Cabernet Sauvignon and Chardonnay and the skins analysed for resveratrol production following irradiation with UV-C light. The rate and maximal level of resveratrol accumulation increased markedly in berries sampled from 1–5 weeks post-flowering and then dramatically declined in maturing berries sampled from 10–16 weeks post-flowering in all cultivars. In berries sampled at 1 and 5 weeks post-flowering, maximal levels of resveratrol accumulation were recorded at incubation periods of 24 and 48 h respectively whereas maximal resveratrol levels were not recorded in week 16 berry skins until 72 h after UV-treatment. Gene expression analysis indicated that stilbene synthase (STS) mRNA accumulated within 4–8 h of UV treatment in berries sampled at 1 and 5 weeks post-flowering, but did not increase in week 16 berries until 24–48 h following UV-irradiation. Furthermore, the overall level of STS gene expression declined in berries sampled 10–16 weeks post-flowering. The results demonstrate that inducible stilbene accumulation in ripening grape berries is highly regulated at the level of STS gene transcription. This decline in inducible STS gene expression may be a major factor contributing to the increased susceptibility of ripening grape berries to Botrytis cinerea infection. |
Keywords: | Botrytis cinerea, grape berry development, resveratrol, stilbene synthase, UV-irradiation, Vitis vinifera |
Rights: | © CSIRO 2000 |
DOI: | 10.1071/PP00007 |
Appears in Collections: | Agriculture, Food and Wine publications |
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