Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/28012
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Eph receptors and ephrins in the developing chick cerebellum: relationship to sagittal patterning and granule cell migration |
Author: | Karam, S. Burrows, R. Logan, C. Koblar, S. Pasquale, E. Bothwell, M. |
Citation: | The Journal of Neuroscience, 2000; 20(17):6488-6500 |
Publisher: | Soc Neuroscience |
Issue Date: | 2000 |
ISSN: | 0270-6474 1529-2401 |
Organisation: | Centre for the Molecular Genetics of Development |
Abstract: | Spatiotemporal expression patterns of six members of the Eph gene family (EphA4, EphA3, EphB2, ephrin-B1, ephrin-A2, and ephrin-A5) were characterized immunocytochemically at various stages of chick cerebellar development. EphA4 expression is observed in the cerebellar anlage as early as embryonic day 5 (E5) and continues in the posthatch cerebellum. During the early period of cerebellar development (E3-E8), complementarity is observed between EphA4 and ephrin-A5 expression within the cerebellar-isthmal region. By E8, differential expression of EphA4 in parasagittal Purkinje cell bands is evident, and the expression remains banded in the posthatch cerebellum. Banded expression of the ephrin-A5 ligand complements EphA4 expression during the middle period (E9-E15). During this period, ephrin-A2 and EphA3 are coexpressed in a banded pattern and with variable correlation to EphA4. Variability in the banding expression is observed for EphA4, EphA3, ephrin-A5, and ephrin-A2 across different lobes, and graded complementarity in the expression pattern of EphA3 and ephrin-A5 is observed in the external granular layer between the posterior and anterior lobes. Analysis of Purkinje cell birth date in correlation with Eph-ephrin expression during the middle period reveals that early-born cells express EphA4, whereas late-born cells express ephrin-A5. Finally, EphA4 expression domains are respected by migrating granule cell ribbons, which express both ephrin-B1 and EphB2. These expression patterns suggest multiple roles for the Eph-ephrin system in cerebellar development, including demarcation/enforcement of boundaries of the cerebellar anlage, formation/maintenance of Purkinje cell compartments, and restriction of the early phase of granule cell migration to ribbons. |
Keywords: | Purkinje cell |
DOI: | 10.1523/jneurosci.20-17-06488.2000 |
Published version: | http://www.jneurosci.org/cgi/content/abstract/20/17/6488 |
Appears in Collections: | Aurora harvest 6 Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_28012.pdf | 2.78 MB | Publisher's PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.