Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/28070
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Type: Journal article
Title: Oral administration of formaldehyde-killed recombinant bacteria expressing a mimic of the shiga toxin receptor protects mice from fatal challenge with shiga-toxigenic Escherichia coli
Author: Paton, J.
Rogers, T.
Morona, R.
Paton, A.
Citation: Infection and Immunity, 2001; 69(3):1389-1393
Publisher: Amer Soc Microbiology
Issue Date: 2001
ISSN: 0019-9567
1098-5522
Statement of
Responsibility: 
James C. Paton, Trisha J. Rogers, Renato Morona, and Adrienne W. Paton
Abstract: Gastrointestinal disease caused by Shiga toxin-producing Escherichia coli (STEC) is frequently complicated by life-threatening toxin-induced systemic sequelae, including the hemolytic uremic syndrome. We previously constructed a recombinant bacterium displaying a Shiga toxin receptor mimic on its surface which neutralized Shiga toxins with very high efficiency. Moreover, oral administration of the live bacterium completely protected mice from challenge with virulent STEC. In this study, we investigated the protective capacity of formaldehyde-killed receptor mimic bacteria, as these are likely to be safer for administration to humans. The killed bacteria completely protected STEC-challenged mice when administered three times daily; incomplete protection was achieved using two doses per day. Commencement of therapy could be delayed for up to 48 h after challenge without diminishing protection, depending on the virulence of the challenge strain. Thus, administration of this agent early in the course of human STEC disease may prevent progression to life-threatening complications.
Keywords: Animals; Mice, Inbred BALB C; Mice; Escherichia coli; Escherichia coli Infections; Hemolytic-Uremic Syndrome; Formaldehyde; Trihexosylceramides; Receptors, Cell Surface; Vaccines, Synthetic; Shiga Toxin; Escherichia coli Vaccines; Vaccines, Inactivated; Administration, Oral; Molecular Mimicry; Male
Description: Copyright © 2001 by the American Society for Microbiology. All rights reserved.
RMID: 0020010646
DOI: 10.1128/IAI.69.3.1389-1393.2001
Appears in Collections:Molecular and Biomedical Science publications

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