Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/30876
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dc.contributor.authorZannettino, A.en
dc.contributor.authorFarrugia, A.en
dc.contributor.authorTo, L.en
dc.contributor.authorAtkins, G.en
dc.date.issued2005en
dc.identifier.citationMultiple Myeloma: Methods and Protocols, 2005 / Brown, R., Joy Ho, P. (ed./s), pp.245-256en
dc.identifier.isbn1592599168en
dc.identifier.urihttp://hdl.handle.net/2440/30876-
dc.description.abstractMuch of the morbidity and mortality associated with the plasma cell (PC) malignancy, multiple myeloma (MM), is owing to the severe osteolytic bone disease seen in patients with this disease. Although the molecular mechanisms responsible for osteolysis remain to be fully elucidated, it is clear from numerous studies that it is owing, in part, to an increase in osteoclastic bone resorption. Several known osteoclast (OC)-activating factors (OAFs) are produced by myeloma PCs (MPCs), or by stromal cells in response to MPCs and include interleukin-1beta (IL-1beta); tumor necrosis factor-alpha (TNF-alpha); IL-6; parathyroid hormone-related protein; macrophage inflammatory protein-1alpha; and, most recently, the TNF-ligand family member receptor activator of nuclear factor-kappaB ligand (RANKL). The identification and significance of any one of these myeloma-derived OAFs is dependent on robust and reliable assays that measure the de novo formation and activation of OCs. A number of in vitro assay systems have been described that examine the requirements for normal OC formation and are easily adaptable for examining which MM-derived OAF and to what extent it is responsible for the bone loss observed in individuals with myeloma. This chapter describes one such in vitro model system.en
dc.language.isoenen
dc.publisherHumana Pressen
dc.subjectBone and Bones; Plasma Cells; Leukocytes, Mononuclear; Osteoclasts; Humans; Multiple Myeloma; Bone Diseases; Bone Resorption; Osteolysis; Antigens, CD14; Antigens, CD; Microscopy, Electron, Scanning; Cell Culture Techniques; Coculture Techniquesen
dc.titleAn in vitro osteoclast-forming assay to measure myeloma cell-derived osteoclast-activating factorsen
dc.typeBook chapteren
dc.identifier.rmid0020052434en
dc.identifier.doi10.1385/1-59259-916-8:245en
dc.publisher.placeTotowa, New Jerseyen
dc.identifier.pubid53645-
pubs.library.collectionOrthopaedics and Trauma publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidZannettino, A. [0000-0002-6646-6167]en
dc.identifier.orcidAtkins, G. [0000-0002-3123-9861]en
Appears in Collections:Orthopaedics and Trauma publications

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