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Type: Journal article
Title: The Conserved, 5' Termini of RNAs 1 and 2 of Tomato aspermy virus are Dispensable for Infection but Affect Virulence
Author: Shi, B.
Palukaitis, P.
Symons, R.
Citation: Virus Genes, 2005; 30(2):181-191
Publisher: Kluwer Academic Publ
Issue Date: 2005
ISSN: 0920-8569
Statement of
Bu-Jun Shi, Peter Palukaitis and Robert H. Symons
Abstract: The 5 terminus of each of the three genomic RNAs (RNAs 1, 2 and 3) of Tomato aspermy virus (TAV) begins with the sequence 5-GUUU, which is also shared by a number of other viruses. Mutagenic analyses showed that the 5-GUUU sequence of RNAs 1 and 2 of TAV was dispensable for viral infection and did not prevent symptom induction. On the other hand, substitution of U at position 5 for G in RNA 1, but not RNA 2, induced veinal necrosis symptoms in Nicotiana glutinosa. The mutants constructed included insertion of UUU into the 5-GUUU sequence of TAV RNAs 1 and/or 2. All RNA 2 mutants induced more severe symptoms than viral RNAs containing either mutated RNA 1 or most combinations of mutated RNAs 1 and 2. Some combinations of mutated RNAs 1 and 2 also induced veinal necrosis in N. glutinosa. Virulence was unrelated to the levels of viral RNA accumulation. Sequence analysis of progeny viral RNAs showed that only the mutant viral RNAs with a G to U substitution in RNA 1 and the deletion of the 5-GUUU in both RNAs 1 and 2 were able to maintain the same sequence as the inoculum. The other mutants either reverted to the wildtype sequence or underwent further deletion or insertion. None of the constructed mutants were able to compete for accumulation with the wildtype virus after co-inoculation to the plant species tested.
Keywords: Cucumovirus; Tobacco; DNA, Viral; RNA, Viral; Virulence; Plant Diseases; Mutagenesis; Base Sequence; Conserved Sequence; RNA Stability
Description: The original publication can be found at
RMID: 0020063395
DOI: 10.1007/s11262-004-5626-1
Published version:
Appears in Collections:Australian Centre for Plant Functional Genomics publications
Agriculture, Food and Wine publications

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