Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/34194
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Genetic and molecular characterization of the maize rp3 rust resistance locus |
Author: | Webb, C. Richter, T. Collins, N. Nicolas, M. Trick,, H. Pryor, A. Hulbert, S. |
Citation: | Genetics: a periodical record of investigations bearing on heredity and variation, 2002; 162(1):381-394 |
Publisher: | Genetics |
Issue Date: | 2002 |
ISSN: | 0016-6731 1943-2631 |
Statement of Responsibility: | Craig A. Webb, Todd E. Richter, Nicholas C. Collins, Marie Nicolas, Harold N. Trick, Tony Pryor and Scot H. Hulbert |
Abstract: | In maize, the Rp3 gene confers resistance to common rust caused by Puccinia sorghi. Flanking marker analysis of rust-susceptible rp3 variants suggested that most of them arose via unequal crossing over, indicating that rp3 is a complex locus like rp1. The PIC13 probe identifies a nucleotide binding site-leucine-rich repeat (NBS-LRR) gene family that maps to the complex. Rp3 variants show losses of PIC13 family members relative to the resistant parents when probed with PIC13, indicating that the Rp3 gene is a member of this family. Gel blots and sequence analysis suggest that at least 9 family members are at the locus in most Rp3-carrying lines and that at least 5 of these are transcribed in the Rp3-A haplotype. The coding regions of 14 family members, isolated from three different Rp3-carrying haplotypes, had DNA sequence identities from 93 to 99%. Partial sequencing of clones of a BAC contig spanning the rp3 locus in the maize inbred line B73 identified five different PIC13 paralogues in a region of ~140 kb. |
Keywords: | Basidiomycota Zea mays Plant Proteins DNA Primers Meiosis Recombination, Genetic Amino Acid Sequence Base Sequence Sequence Homology, Amino Acid Haplotypes Phenotype Genes, Plant Molecular Sequence Data |
Rights: | Copyright © 2002 by the Genetics Society of America |
DOI: | 10.1093/genetics/162.1.381 |
Published version: | http://www.genetics.org/cgi/content/abstract/162/1/381 |
Appears in Collections: | Aurora harvest Australian Centre for Plant Functional Genomics publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.