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|Title:||Genetic and molecular characterization of the maize rp3 rust resistance locus|
|Citation:||Genetics, 2002; 162(1):381-394|
|Craig A. Webb, Todd E. Richter, Nicholas C. Collins, Marie Nicolas, Harold N. Trick, Tony Pryor and Scot H. Hulbert|
|Abstract:||In maize, the Rp3 gene confers resistance to common rust caused by Puccinia sorghi. Flanking marker analysis of rust-susceptible rp3 variants suggested that most of them arose via unequal crossing over, indicating that rp3 is a complex locus like rp1. The PIC13 probe identifies a nucleotide binding site-leucine-rich repeat (NBS-LRR) gene family that maps to the complex. Rp3 variants show losses of PIC13 family members relative to the resistant parents when probed with PIC13, indicating that the Rp3 gene is a member of this family. Gel blots and sequence analysis suggest that at least 9 family members are at the locus in most Rp3-carrying lines and that at least 5 of these are transcribed in the Rp3-A haplotype. The coding regions of 14 family members, isolated from three different Rp3-carrying haplotypes, had DNA sequence identities from 93 to 99%. Partial sequencing of clones of a BAC contig spanning the rp3 locus in the maize inbred line B73 identified five different PIC13 paralogues in a region of ~140 kb.|
|Keywords:||Basidiomycota; Zea mays; Plant Proteins; DNA Primers; Meiosis; Recombination, Genetic; Amino Acid Sequence; Base Sequence; Sequence Homology, Amino Acid; Haplotypes; Phenotype; Genes, Plant; Molecular Sequence Data|
|Rights:||Copyright © 2002 by the Genetics Society of America|
|Appears in Collections:||Australian Centre for Plant Functional Genomics publications|
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