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https://hdl.handle.net/2440/40046
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Type: | Journal article |
Title: | Growth factors and cytokines modulate gene expression of cell-surface proteoglycans in human periodontal ligament cells |
Author: | Worapamorn, W. Haase, H. Li, H. Bartold, P. |
Citation: | Journal of Cellular Physiology, 2001; 186(3):448-456 |
Publisher: | Wiley-Liss |
Issue Date: | 2001 |
ISSN: | 0021-9541 1097-4652 |
Statement of Responsibility: | W. Worapamorn, H.R. Haase, H. Li, and P.M. Bartold |
Abstract: | Cell-surface proteoglycans have been known to be involved in many functions including interactions with components of the extracellular microenvironment, and act as co-receptors which bind and modify the action of various growth factors and cytokines. The purpose of this study was to determine the regulation by growth factors and cytokines on cell-surface proteoglycan gene expression in cultured human periodontal ligament (PDL) cells. Subconfluent, quiescent PDL cells were treated with various concentrations of serum, bFGF, PDGF-BB, TGF-beta1, IL-beta1, and IFN-gamma. RT-PCR technique was used, complemented with Northern blot for syndecan-1, to examine the effects of these agents on the mRNA expression of five cell-surface proteoglycans (syndecan-1, syndecan-2, syndecan-4, glypican and betaglycan). Syndecan-1 mRNA levels increased in response to serum, bFGF and PDGF-BB, but decreased in response to TGF-beta1, IL-1beta and IFN-gamma. In contrast, syndecan-2 mRNA levels were upregulated by TGF-beta1 and IL-1beta stimulation, but remained unchanged with the other agents. Betaglycan gene expression decreased in response to serum, but was upregulated by TGF-beta1 and unchanged by the other stimulants. Additionally, syndecan-4 and glypican were not significantly altered in response to the regulator molecules studied, with the exception that glypican is decreased in response to IFN-gamma. These data demonstrate that the gene expression of the five cell-surface proteoglycans studied is differentially regulated in PDL cells lending support to the notion of distinct functions for these cell-surface proteoglycans. |
Keywords: | Cells, Cultured Cell Membrane Periodontal Ligament Humans Proteoglycans Growth Substances Fibroblast Growth Factor 2 Platelet-Derived Growth Factor Proto-Oncogene Proteins c-sis Transforming Growth Factor beta beta 2-Microglobulin Membrane Glycoproteins Receptors, Transforming Growth Factor beta Recombinant Proteins Interleukin-1 Cytokines Reverse Transcriptase Polymerase Chain Reaction Cell Division Cell Movement Transcription, Genetic Gene Expression Regulation Heparan Sulfate Proteoglycans Syndecan-1 Syndecans Syndecan-4 Syndecan-2 Interferon-gamma Becaplermin |
Description: | Published online in Wiley InterScience, 23 January 2001 |
Rights: | © 2001 Wiley-Liss, Inc. |
DOI: | 10.1002/1097-4652(2001)9999:9999<000::AID-JCP1047>3.0.CO;2-V |
Published version: | http://onlinelibrary.wiley.com/doi/10.1002/1097-4652%282001%299999:9999%3C000::AID-JCP1047%3E3.0.CO;2-V/abstract |
Appears in Collections: | Aurora harvest 6 Dentistry publications |
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