Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/41469
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Type: Journal article
Title: Maternal supply of omega 3 polyunsaturated fatty acids alter mechanisms involved in oocyte and early embryo development in the mouse
Author: Wakefield, S.
Lane, M.
Schulz, S.
Hebart, M.
Thompson, J.
Mitchell, M.
Citation: American Journal of Physiology-Endocrinology and Metabolism, 2008; 294(2):E425-E434
Publisher: Amer Physiological Soc
Issue Date: 2008
ISSN: 0193-1849
1522-1555
Statement of
Responsibility: 
Sarah L. Wakefield, Michelle Lane, Samantha J. Schulz, Michelle L. Hebart, Jeremy G. Thompson, and Megan Mitchell
Abstract: Despite the well-known benefits of omega-3 (n-3) polyunsaturated fatty acid (PUFA) supplementation on human health, relatively little is known about the effect of n-3 PUFA intake on fertility. More specifically, the aim of this study was to determine how oocyte and preimplantation embryo development might be influenced by n-3 PUFA supply and to understand the possible mechanisms underlying these effects. Adult female mice were fed a control diet or a diet relatively high in the long-chain n-3 PUFAs for 4 wk, and ovulated oocytes or zygotes were collected after gonadotropin stimulation. Oocytes were examined for mitochondrial parameters (active mitochondrial distribution, mitochondrial calcium and membrane potential) and oxidative stress, and embryo developmental ability was assessed at the blastocyst stage following 1) in vitro fertilization (IVF) or 2) culture of in vivo-derived zygotes. This study demonstrated that exposure of the oocyte during maturation in the ovary to an environment high in n-3 PUFA resulted in altered mitochondrial distribution and calcium levels and increased production of reactive oxygen species. Despite normal fertilization and development in vitro following IVF, the exposure of oocytes to an environment high in n-3 PUFA during in vivo fertilization adversely affected the morphological appearance of the embryo and decreased developmental ability to the blastocyst stage. This study suggests that high maternal dietary n-3 PUFA exposure periconception reduces normal embryo development in the mouse and is associated with perturbed mitochondrial metabolism, raising questions regarding supplementation with n-3 PUFAs during this period of time.
Keywords: Oocytes; Zygote; Mitochondria; Animals; Mice, Inbred C57BL; Mice; Body Weight; Calcium; Reactive Oxygen Species; Fatty Acids, Omega-3; Fatty Acids; RNA; Antioxidants; Organ Size; Fertilization in Vitro; Embryo Culture Techniques; Diet; Reverse Transcriptase Polymerase Chain Reaction; Membrane Potentials; Oxidative Stress; Embryonic Development; Pregnancy; Female
Rights: Copyright © 2007 by the American Physiological Society.
RMID: 0020073617
DOI: 10.1152/ajpendo.00409.2007
Published version: http://ajpendo.physiology.org/cgi/content/abstract/294/2/E425
Appears in Collections:Obstetrics and Gynaecology publications

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