Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/41487
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Type: Journal article
Title: Grapes on Steroids. Brassinosteroids Are Involved in Grape Berry Ripening
Author: Symons, G.
Davies, C.
Shavrukov, Y.
Dry, I.
Reid, J.
Thomas, M.
Citation: Plant Physiology, 2006; 140(1):150-158
Publisher: Amer Soc Plant Physiologists
Issue Date: 2006
ISSN: 0032-0889
1532-2548
Organisation: Australian Centre for Plant Functional Genomics (ACPFG)
Abstract: Fruit ripening is a unique plant developmental process with direct implications for our food supply, nutrition, and health. In contrast to climacteric fruit, where ethylene is pivotal, the hormonal control of ripening in nonclimacteric fruit, such as grape (Vitis vinifera), is poorly understood. Brassinosteroids (BRs) are steroidal hormones, essential for normal plant growth and development but not previously implicated in the ripening of nonclimacteric fruit. Here we show that increases in endogenous BR levels, but not indole-3-acetic acid (IAA) or GA levels, are associated with ripening in grapes. Putative grape homologs of genes encoding BR biosynthesis enzymes (BRASSINOSTEROID-6-OXIDASE and DWARF1) and the BR receptor (BRASSINOSTEROID INSENSITIVE 1) were isolated, and the function of the grape BRASSINOSTEROID-6-OXIDASE gene was confirmed by transgenic complementation of the tomato (Lycopersicon esculentum) extreme dwarf (dx/dx) mutant. Expression analysis of these genes during berry development revealed transcript accumulation patterns that were consistent with a dramatic increase in endogenous BR levels observed at the onset of fruit ripening. Furthermore, we show that application of BRs to grape berries significantly promoted ripening, while brassinazole, an inhibitor of BR biosynthesis, significantly delayed fruit ripening. These results provide evidence that changes in endogenous BR levels influence this key developmental process. This may provide a significant insight into the mechanism controlling ripening in grapes, which has direct implications for the logistics of grape production and down-stream processing.
Keywords: Plants, Genetically Modified; Lycopersicon esculentum; Vitis; Gibberellins; Indoleacetic Acids; Triazoles; Steroids; Plant Growth Regulators; Plant Proteins; Cloning, Molecular; Phylogeny; Signal Transduction; Gene Expression Regulation, Plant; Molecular Sequence Data
RMID: 0020075453
DOI: 10.1104/pp.105.070706
Description (link): http://stke.sciencemag.org/cgi/content/abstract/plantphysiol;140/1/150
Appears in Collections:Australian Centre for Plant Functional Genomics publications

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