Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/42946
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Type: Journal article
Title: The proteomic profile of Fusobacterium nucleatum is regulated by growth pH
Author: Zilm, P.
Bagley, C.
Rogers, A.
Milne, I.
Gully, N.
Citation: Microbiology, 2007; 153 Part 1(1):148-159
Publisher: Soc General Microbiology
Issue Date: 2007
ISSN: 1350-0872
1465-2080
Statement of
Responsibility: 
Peter S. Zilm, Christopher J. Bagle, Anthony H. Rogers, Ian R. Milne and Neville J. Gully
Abstract: Fusobacterium nucleatum is a saccharolytic Gram-negative anaerobic organism believed to play an important role in the microbial succession associated with the development of periodontal disease. Its genome contains niche-specific genes shared with the other inhabitants of dental plaque, which may help to explain its ability to survive and grow in the changing environmental conditions experienced in the gingival sulcus during the transition from health to disease. The pH of the gingival sulcus increases during the development of periodontitis and this is thought to occur by the metabolism of nutrients supplied by gingival crevicular fluid. In comparison with other plaque inhabitants, F. nucleatum has the greatest ability to neutralize acidic environments. The differential expression of soluble cytoplasmic proteins induced by acidic (pH 6.4) or basic (pH 7.4 and 7.8) conditions, during long-term anaerobic growth in a chemostat, was identified by two-dimensional gel electrophoresis and image analysis software. Twenty-two proteins, found to have altered expression in response to external pH, were identified by tryptic digestion and mass spectrometry. Eight differentially expressed proteins associated with increased energy (ATP) production via the 2-oxoglutarate and Embden–Meyerhof pathways appeared to be directed towards either cellular biosynthesis or the maintenance of internal homeostasis. Overall, these results represent the first proteomic investigation of F. nucleatum and the identification of gene products which may be important in the organism's persistence during the transition from health to disease in vivo.
Keywords: Fusobacterium nucleatum
Bacterial Proteins
Culture Media
Electrophoresis, Gel, Two-Dimensional
Proteomics
Anaerobiosis
Hydrogen-Ion Concentration
Description: Copyright © 2007 Society for General Microbiology
DOI: 10.1099/mic.0.2006/001040-0
Published version: http://dx.doi.org/10.1099/mic.0.2006/001040-0
Appears in Collections:Aurora harvest 6
Dentistry publications

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