Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/43046
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Type: Journal article
Title: Identification of Photosystem I and Photosystem II enriched regions of thylakoid membrane by optical microimaging of cryo-fluorescence emission spectra and of variable fluorescence
Author: Vacha, F.
Sarafis, Vassilios
Benediktyova, Z.
Bumba, L.
Valenta, J.
Vacha, M.
Sheue, Chiou-Rong
Nedbal, L.
Citation: Micron, 2007; 38(2):170-175
Publisher: Pergamon-Elsevier Science Ltd
Issue Date: 2007
ISSN: 0968-4328
School/Discipline: School of Medical Sciences : Anatomical Sciences
Abstract: Oxygenic photosynthesis of higher plants requires linear electron transport that is driven by serially operating Photosystem II and Photosystem I reaction centers. It is widely accepted that distribution of these two types of reaction centers in the thylakoid membrane is heterogeneous. Here, we describe two optical microscopic techniques that can be combined to reveal the heterogeneity. By imaging micro-spectroscopy at liquid nitrogen temperature, we resolved the heterogeneity of the chloroplast thylakoid membrane by distinct spectral signatures of fluorescence emitted by the two photosystems. With another microscope, we measured changes in the fluorescence emission yield that are induced by actinic light at room temperature. Fluorescence yield of Photosystem II reaction centers varies strongly with light-induced changes of its photochemical yield. Consequently, application of moderate background irradiance induces changes in the Photosystem II fluorescence yield whereas no such modulation occurs in Photosystem I. This contrasting feature was used to identify regions in thylakoid membranes that are enriched in active Photosystem II.
Keywords: Chloroplast; Heterogeneity; Microscopy; Photosynthesis
Rights: Copyright © Elsevier Ltd.
DOI: 10.1016/j.micron.2006.07.013
Description (link): http://www.sciencedirect.com/science/journal/09684328
Appears in Collections:Anatomical Sciences publications

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