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https://hdl.handle.net/2440/43836
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Type: | Journal article |
Title: | Airway infection in stable lung transplant patients is associated with decreased intracellular T-helper type 1 pro-inflammatory cytokines in bronchoalveolar lavage T-cell subsets |
Author: | Hodge, G. Hodge, S. Reynolds, P. Holmes, M. |
Citation: | Transplant Infectious Disease, 2008; 10(2):99-105 |
Publisher: | Wiley-Blackwell Munksgaard |
Issue Date: | 2008 |
ISSN: | 1399-3062 1399-3062 |
Statement of Responsibility: | G. Hodge, S. Hodge, P.N. Reynolds and M. Holmes |
Abstract: | Current immunosuppression protocols to prevent lung transplant rejection reduce pro-inflammatory and T-helper type 1 (Th1) cytokines. However, Th1 T-cell pro-inflammatory cytokine production is important in host defense against bacterial infection in the lungs. Excessive immunosuppression of Th1 T-cell pro-inflammatory cytokines leaves patients susceptible to infection. To investigate whether pulmonary infection in lung transplant recipients is associated with reduced Th1 T-cell pro-inflammatory cytokines, whole blood and bronchoalveolar lavage (BAL) fluid from 13 stable lung transplant patients with ‘culture-negative’ BAL and 13 patients with ‘culture-positive’ BAL was stimulated in vitro, and cytokine production by CD8+ and CD4+ T-cell subsets was determined using multiparameter flow cytometry. In BAL samples, there was a significant decrease in interleukin-2 (IL2) in CD3+ T cells and tumor necrosis factor-α (TNF-α) in CD8+ T cells (but not CD4+) in ‘culture-positive’ compared with ‘culture-negative’ transplant patients. There was no difference in blood Th1 T-cell cytokines between ‘culture-positive’ compared with ‘culture-negative’ transplant patients. A decrease in Th1 cytokines IL-2 and TNF-α in BAL T-cell subsets is associated with isolation of potentially pathogenic organisms in the lungs in stable lung transplant patients. Excessive immunosuppression of these Th1 T-cell pro-inflammatory cytokines in stable transplant patients may leave them susceptible to infection. Modifying immunosuppression by monitoring intracellular Th1 pro-inflammatory cytokines in BAL T cells may help to improve morbidity and infection rates in stable lung transplant patients. |
Keywords: | T-Lymphocyte Subsets T-Lymphocytes CD4-Positive T-Lymphocytes Th1 Cells CD8-Positive T-Lymphocytes Bronchoalveolar Lavage Fluid Humans Lung Diseases Tumor Necrosis Factor-alpha Interleukin-2 Cytokines Bronchoscopy Lung Transplantation Flow Cytometry Case-Control Studies Transplantation Immunology Immunocompromised Host Adult Female Male |
Description: | The definitive version is available at www.blackwell-synergy.com |
Provenance: | Published online in 2007. |
DOI: | 10.1111/j.1399-3062.2007.00236.x |
Published version: | http://dx.doi.org/10.1111/j.1399-3062.2007.00236.x |
Appears in Collections: | Aurora harvest Paediatrics publications |
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