Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/44700
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Type: Journal article
Title: The effect of microbial inoculants on the indigenous actinobacterial endophyte population present in the roots of wheat as determined by Terminal Restriction Fragment Length Polymorphism
Author: Conn, V.
Franco, C.
Citation: Applied and Environmental Microbiology, 2004; 70(11):6407-6413
Publisher: Amer Soc Microbiology
Issue Date: 2004
ISSN: 0099-2240
Organisation: Australian Centre for Plant Functional Genomics (ACPFG)
Abstract: The effect of single actinobacterial endophyte seed inoculants and a mixed microbial soil inoculant on the indigenous endophytic actinobacterial population in wheat roots was investigated by using the molecular technique terminal restriction fragment length polymorphism (T-RFLP). Wheat was cultivated either from seeds coated with the spores of single pure actinobacterial endophytes of Microbispora sp. strain EN2, Streptomyces sp. strain EN27, and Nocardioides albus EN46 or from untreated seeds sown in soil with and without a commercial mixed microbial soil inoculant. The endophytic actinobacterial population within the roots of 6-week-old wheat plants was assessed by T-RFLP. Colonization of the wheat roots by the inoculated actinobacterial endophytes was detected by T-RFLP, as were 28 to 42 indigenous actinobacterial genera present in the inoculated and uninoculated plants. The presence of the commercial mixed inoculant in the soil reduced the endophytic actinobacterial diversity from 40 genera to 21 genera and reduced the detectable root colonization by approximately half. The results indicate that the addition of a nonadapted microbial inoculum to the soil disrupted the natural actinobacterial endophyte population, reducing diversity and colonization levels. This was in contrast to the addition of a single actinobacterial endophyte to the wheat plant, where the increase in colonization level could be confirmed even though the indigenous endophyte population was not adversely affected.
RMID: 0020077406
DOI: 10.1128/AEM.70.11.6407-6413.2004
Appears in Collections:Australian Centre for Plant Functional Genomics publications

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