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Type: Journal article
Title: The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Author: Fleury, D.
Himanen, K.
Cnops, G.
Nelissen, H.
Boccardi, T.
Maere, S.
Beemster, G.
Neyt, P.
Anami, S.
Robels, P.
Micol, J.
Inze, D.
Van Lijsebettens, M.
Citation: Plant Cell, 2007; 19(2):417-432
Publisher: Amer Soc Plant Physiologists
Issue Date: 2007
ISSN: 1040-4651
Organisation: Australian Centre for Plant Functional Genomics (ACPFG)
Abstract: Chromatin modification and transcriptional activation are novel roles for E3 ubiquitin ligase proteins that have been mainly associated with ubiquitin-dependent proteolysis. We identified HISTONE MONOUBIQUITINATION1 (HUB1) (and its homolog HUB2) in Arabidopsis thaliana as RING E3 ligase proteins with a function in organ growth. We show that HUB1 is a functional homolog of the human and yeast BRE1 proteins because it monoubiquitinated histone H2B in an in vitro assay. Hub knockdown mutants had pale leaf coloration, modified leaf shape, reduced rosette biomass, and inhibited primary root growth. One of the alleles had been designated previously as ang4-1. Kinematic analysis of leaf and root growth together with flow cytometry revealed defects in cell cycle activities. The hub1-1 (ang4-1) mutation increased cell cycle duration in young leaves and caused an early entry into the endocycles. Transcript profiling of shoot apical tissues of hub1-1 (ang4-1) indicated that key regulators of the G2-to-M transition were misexpressed. Based on the mutant characterization, we postulate that HUB1 mediates gene activation and cell cycle regulation probably through chromatin modifications.
Keywords: Humans; Arabidopsis; Plant Leaves; Plant Roots; Ligases; Ubiquitin-Protein Ligase Complexes; Ubiquitin-Protein Ligases; Saccharomyces cerevisiae Proteins; Arabidopsis Proteins; Ubiquitin; Oligonucleotide Array Sequence Analysis; Gene Expression Profiling; Cell Cycle; Cell Proliferation; Gene Expression Regulation, Plant; Phenotype; Molecular Sequence Data; Transcriptional Activation
RMID: 0020077422
DOI: 10.1105/tpc.106.041319
Appears in Collections:Australian Centre for Plant Functional Genomics publications

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