Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/45131
Type: Book chapter
Title: Insertional Mutagenesis: lessons from Arabidopsis, gaining experience in rice
Author: Guiderdoni, Emmanuel
Piffanelli, Pietro
Sallaud, Christophe
Bourgeois, Emmanuelle
Johnson, Alexander Arthur Theodore
Citation: Proceedings of the International Congress "In the Wake of the Double Helix: From the Green Revolution to the Gene Revolution", 27-31 May, 2003 / Tuberosa R., Phillips R.L., Gale M. (eds.): pp.379-402
Publisher: Avenue Media
Issue Date: 2005
ISBN: 8886817487
Organisation: Australian Centre for Plant Functional Genomics (ACPFG)
Abstract: Insertional mutagenesis is a means of analyzing the function of genes through their disruption by foreign DNA followed by observation of the effect on phenotype. Insertion libraries are a key tool in plant functional genomics projects aimed at deciphering major developmental and physiological processes because they permit both the discovery of new genes through forward genetics screens and gene detection systems as well as the validation of genes of known sequence through reverse genetics strategies. Benefiting from experience accumulated over the last decade in the model dicotyledonous species Arabidopsis, large insertion line libraries in turn have been generated in rice (Oryza sativa L.), the model plant for cereals and grasses, in the last five years. The use of insertion mutagens has varied and encompasses T-DNA, delivered during co-culture with Agrobacterium, the genetically engineered maize transposable Ac/Ds system and the endogenous ty1-copia retroelement Tos17, mobilized during tissue culture. Here we review the current status of rice insertional mutagenesis by concentrating on specific features and advantages and drawbacks of each mutagen, and examine the establishment of this new resource from a rice functional genomics perspective.
RMID: 0020077509
Description (link): http://aob.oxfordjournals.org/cgi/content/full/98/4/900
Appears in Collections:Australian Centre for Plant Functional Genomics publications

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