Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/45131
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dc.contributor.authorGuiderdoni, Emmanuelen
dc.contributor.authorPiffanelli, Pietroen
dc.contributor.authorSallaud, Christopheen
dc.contributor.authorBourgeois, Emmanuelleen
dc.contributor.authorJohnson, Alexander Arthur Theodoreen
dc.date.issued2005en
dc.identifier.citationProceedings of the International Congress "In the Wake of the Double Helix: From the Green Revolution to the Gene Revolution", 27-31 May, 2003 / Tuberosa R., Phillips R.L., Gale M. (eds.): pp.379-402en
dc.identifier.isbn8886817487en
dc.identifier.urihttp://hdl.handle.net/2440/45131-
dc.description.abstractInsertional mutagenesis is a means of analyzing the function of genes through their disruption by foreign DNA followed by observation of the effect on phenotype. Insertion libraries are a key tool in plant functional genomics projects aimed at deciphering major developmental and physiological processes because they permit both the discovery of new genes through forward genetics screens and gene detection systems as well as the validation of genes of known sequence through reverse genetics strategies. Benefiting from experience accumulated over the last decade in the model dicotyledonous species Arabidopsis, large insertion line libraries in turn have been generated in rice (Oryza sativa L.), the model plant for cereals and grasses, in the last five years. The use of insertion mutagens has varied and encompasses T-DNA, delivered during co-culture with Agrobacterium, the genetically engineered maize transposable Ac/Ds system and the endogenous ty1-copia retroelement Tos17, mobilized during tissue culture. Here we review the current status of rice insertional mutagenesis by concentrating on specific features and advantages and drawbacks of each mutagen, and examine the establishment of this new resource from a rice functional genomics perspective.en
dc.description.urihttp://aob.oxfordjournals.org/cgi/content/full/98/4/900en
dc.publisherAvenue Mediaen
dc.titleInsertional Mutagenesis: lessons from Arabidopsis, gaining experience in riceen
dc.typeBook chapteren
dc.identifier.rmid0020077509en
dc.contributor.organisationAustralian Centre for Plant Functional Genomics (ACPFG)en
Appears in Collections:Australian Centre for Plant Functional Genomics publications

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