Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/46228
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorLi, M.en
dc.contributor.authorSingh, R.en
dc.contributor.authorBazanova, N.en
dc.contributor.authorMilligan, A.en
dc.contributor.authorShirley, N.en
dc.contributor.authorLangridge, P.en
dc.contributor.authorLopato, S.en
dc.date.issued2008en
dc.identifier.citationPlant Biotechnology Journal, 2008; 6(5):465-476en
dc.identifier.issn1467-7644en
dc.identifier.issn1467-7652en
dc.identifier.urihttp://hdl.handle.net/2440/46228-
dc.descriptionPublished in Plant Biotechnology, 2008; 6 (5) at www.interscience.wiley.comen
dc.description.abstractTwo putative endosperm-specific rice genes, OsPR602 and OsPR9a, were identified from database searches. The promoter regions of these genes were isolated, and transcriptional promoter:beta-glucuronidase (GUS) fusion constructs were stably transformed into rice and barley. The GUS expression patterns revealed that these promoters were active in early grain development in both rice and barley, and showed strongest expression in endosperm transfer cells during the early stages of grain filling. The GUS expression was similar in both rice and barley, but, in barley, expression was exclusively in the endosperm transfer cells and differed in timing of activation relative to rice. In rice, both promoters showed activity not only in the endosperm transfer cells, but also in the transfer cells of maternal tissue and in several floral tissues shortly before pollination. The expression patterns of OsPR602 and OsPR9a in flowers differed. The similarity of expression in both rice and barley suggests that these promoters may be useful to control transgene expression in the transfer cells of cereal grains with the aim of altering nutrient uptake or enhancing the barrier against pathogens at the boundary between maternal tissue and the developing endosperm. However, the expression during floral development should be considered if the promoters are used in rice.en
dc.description.statementofresponsibilityMing Li, Rohan Singh, Natalia Bazanova, Andrew S. Milligan, Neil Shirley, Peter Langridge and Sergiy Lopatoen
dc.language.isoenen
dc.publisherBlackwell Publishing Ltd.en
dc.source.urihttp://www3.interscience.wiley.com/journal/119421133/abstracten
dc.subjectbarley; endosperm; grain; promoter; rice; transfer cellsen
dc.titleSpatial and temporal expression of endosperm transfer cell-specific promoters in transgenic rice and barleyen
dc.typeJournal articleen
dc.identifier.rmid0020080710en
dc.identifier.doi10.1111/j.1467-7652.2008.00333.xen
dc.identifier.pubid43367-
pubs.library.collectionAgriculture, Food and Wine publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidShirley, N. [0000-0001-8114-9891]en
dc.identifier.orcidLangridge, P. [0000-0001-9494-400X]en
Appears in Collections:Agriculture, Food and Wine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.