Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/46967
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Type: Journal article
Title: Structural and expression analysis of the porcine FUS2 gene
Author: Gatphayak, K.
Knorr, C.
Chen, K.
Brenig, B.
Citation: Gene, 2004; 337(4):105-111
Publisher: Elsevier Science BV
Issue Date: 2004
ISSN: 0378-1119
Abstract: The putative porcine tumor suppressor gene FUS2 or N-acetyltransferase (Nat6) assigned to SSC13q21 spans 864-basepairs (bp) of genomic DNA, consisting of a single exon encoding a protein of 288 amino acids (aa), with 73% identity to the human and 74% to the mouse protein. Similar to man and mouse, the gene possesses an N-acetyltransferase domain, but the cell attachment motif arginine–glycine–aspartate (RGD) is exclusively found in the pig gene. Expression studies of the gene in several organs by RT-amplification and by quantitative polymerase chain reaction (Q-PCR) showed that FUS2 is widely expressed in porcine tissues. A point mutation was detected at position 836 of the coding sequence (G to A) leading to an amino acid substitution from cystein (C) to tyrosine (Y) at position 278 of the protein. Genes of the tumor suppressor gene (TSG) cluster act together to suppress tumor growth through their functional activation of tumor suppressing pathways. Studies in humans have proven that mutations in N-acetyltransferase genes are associated with some kind of cancers. Knowledge of structure and function of the respective porcine genes and proteins is important. Pigs—in particular minipigs—will be the non-rodent biomodels for human oncology and cancer therapy in the future.
Keywords: Acetyltransferase; FUS2; Porcine; TSG; SSC13q21
RMID: 0020082423
DOI: 10.1016/j.gene.2004.04.017
Description (link): http://www.sciencedirect.com/science/journal/03781119
Appears in Collections:Earth and Environmental Sciences publications
Environment Institute publications

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