Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/50626
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Type: Journal article
Title: Sab, a novel autotransporter of locus of enterocyte effacement-negative shiga-toxigenic escherichia coli O113:H21, Contributes to adherence and biofilm formation
Author: Herold, S.
Paton, J.
Paton, A.
Citation: Infection and Immunity, 2009; 77(8):3234-3243
Publisher: Amer Soc Microbiology
Issue Date: 2009
ISSN: 0019-9567
1098-5522
Statement of
Responsibility: 
Sylvia Herold, James C. Paton, and Adrienne W. Paton
Abstract: Shiga-toxigenic Escherichia coli (STEC) strains cause serious gastrointestinal disease, which can lead to potentially life-threatening systemic complications such as hemolytic-uremic syndrome. Although the production of Shiga toxin has been considered to be the main virulence trait of STEC for many years, the capacity to colonize the host intestinal epithelium is a crucial step in pathogenesis. In this study, we have characterized a novel megaplasmid-encoded outer membrane protein in locus of enterocyte effacement (LEE)-negative O113:H21 STEC strain 98NK2, termed Sab (for STEC autotransporter [AT] contributing to biofilm formation). The 4,296-bp sab gene encodes a 1,431-amino-acid protein with the features of members of the AT protein family. When expressed in E. coli JM109, Sab contributed to the diffuse adherence to human epithelial (HEp-2) cells and promoted biofilm formation on polystyrene surfaces. A 98NK2 sab deletion mutant was also defective in biofilm formation relative to its otherwise isogenic wild-type parent, and this was complemented by transformation with a sab-carrying plasmid. Interestingly, an unrelated O113:H21 STEC isolate that had a naturally occurring deletion in sab was similarly defective in biofilm formation. PCR analysis indicated that sab is present in LEE-negative STEC strains belonging to serotypes/groups O113:H21, O23, and O82:H8. These findings raise the possibility that Sab may contribute to colonization in a subset of LEE-negative STEC strains.
Keywords: Cell Line; Epithelial Cells; Animals; Mice, Inbred BALB C; Humans; Mice; Biofilms; Bacterial Outer Membrane Proteins; Escherichia coli Proteins; Membrane Transport Proteins; Phosphoproteins; Virulence Factors; Genetic Complementation Test; Bacterial Adhesion; Gene Deletion; Gene Order; Plasmids; Male; Shiga-Toxigenic Escherichia coli
Description: Copyright © 2009, American Society for Microbiology. All Rights Reserved.
RMID: 0020091499
DOI: 10.1128/IAI.00031-09
Appears in Collections:Molecular and Biomedical Science publications

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