Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/53436
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Type: Journal article
Title: Changes in distribution of basic nuclear proteins and chromatin organization during spermiogenesis in the greater bandicoot rat, Bandicota indica
Author: Worawittayawong, P.
Leigh, C.
Weerachatyanukul, W.
Manochantr, S.
Sobhon, P.
Breed, W.
Sretarugsa, P.
Citation: Cell and Tissue Research, 2008; 334(1):135-144
Publisher: Springer
Issue Date: 2008
ISSN: 0302-766X
1432-0878
Statement of
Responsibility: 
Pakawadee Worawittayawong, Chris Leigh, Wattana Weerachatyanukul, Sirikul Manochantr, Prasert Sobhon, William G. Breed and Prapee Sretarugsa
Abstract: Male germ cells of the greater bandicoot rat, Bandicota indica, have recently been categorized into 12 spermiogenic steps based upon the morphological appearance of the acrosome and nucleus and the cell shape. In the present study, we have found that, in the Golgi and cap phases, round spermatid nuclei contain 10-nm to 30-nm chromatin fibers, and that the acrosomal granule forms a huge cap over the anterior pole of nucleus. In the acrosomal phase, many chromatin fibers are ∼50 nm thick; these then thickened to 70-nm fibers and eventually became 90-nm chromatin cords that are tightly packed together into highly condensed chromatin, except where nuclear vacuoles occur. Immunocytochemistry and immunogold localization with anti-histones, anti-transition protein2, and anti-protamine antibodies suggest that histones remain throughout spermiogenesis, that transition proteins are present from step 7 spermatids and remain until the end of spermiogenesis, and that protamines appear at step 8. Spermatozoa from the cauda epididymidis have been analyzed by acid urea Triton X-100 polyacrylamide gel electrophoresis for basic nuclear proteins. The histones, H2A, H3, H2B, and H4, transitional protein2, and protamine are all present in sperm extracts. These findings suggest that, in these sperm of unusual morphology, both transition proteins and some histones are retained, a finding possibly related to the unusual nuclear form of sperm in this species.
Keywords: Chromatin organization; Sperm basic nuclear proteins; Immunocytochemistry; Spermiogenesis; Bandicota indica (Rodentia)
Description: The original publication can be found at www.springerlink.com
RMID: 0020082288
DOI: 10.1007/s00441-008-0668-7
Appears in Collections:Anatomical Sciences publications
Environment Institute publications

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