Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/54191
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Type: Journal article
Title: Regulation of the divalent metal ion transporter DMT1 and iron homeostasis by a ubiquitin-dependent mechanism involving Ndfips and WWP2
Author: Foot, N.
Dalton, H.
Shearwin-Whyatt, L.
Dorstyn, L.
Tan, S.
Yang, B.
Kumar, S.
Citation: Blood, 2008; 112(10):4268-4275
Publisher: Amer Soc Hematology
Issue Date: 2008
ISSN: 0006-4971
1528-0020
Statement of
Responsibility: 
Foot NJ, Dalton HE, Shearwin-Whyatt LM, Dorstyn L, Tan SS, Yang B and Kumar S.
Abstract: Many ion channels and transporters are regulated by ubiquitination mediated by the Nedd4 family of HECT-type ubiquitin ligases (E3s). These E3s commonly interact with substrates via their WW domains that bind to specific motifs in target proteins. However, not all potential targets of these E3s contain WW-binding motifs. Therefore, accessory proteins may mediate the interaction between Nedd4 family members and their targets. Here we report that the divalent metal ion transporter DMT1, the primary nonheme iron transporter in mammals, is regulated by ubiquitination mediated by the Nedd4 family member WWP2. DMT1 interacts with 2 WW domain-interacting proteins, Ndfip1 and Ndfip2, previously proposed to have roles in protein trafficking. This promotes DMT1 ubiquitination and degradation by WWP2. Consistent with these observations, Ndfip1(-/-) mice show increased DMT1 activity and a concomitant increase in hepatic iron deposition, indicating an essential function of Ndfip1 in iron homeostasis. This novel mechanism of regulating iron homeostasis suggests that Ndfips and WWP2 may contribute to diseases involving aberrant iron transport.
Keywords: CHO Cells; Animals; Mice, Knockout; Cricetulus; Mice; Iron; Ubiquitin-Protein Ligases; Carrier Proteins; Cation Transport Proteins; Membrane Proteins; Ion Transport; Amino Acid Motifs; Protein Structure, Tertiary; Homeostasis; Female; Cricetinae; Male; Ubiquitination; Endosomal Sorting Complexes Required for Transport
RMID: 0020084827
DOI: 10.1182/blood-2008-04-150953
Description (link): http://www.ncbi.nlm.nih.gov/pubmed/18776082
Appears in Collections:Medicine publications

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