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Type: Journal article
Title: Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore Zinquin
Author: Zalewski, P.
Jian, X.
Soon, L.
Breed, W.
Seamark, R.
Lincoln, S.
Ward, A.
Sun, F.Z.
Citation: Reproduction Fertility and Development, 1996; 8(7):1097-1105
Publisher: CSIRO Publishing
Issue Date: 1996
ISSN: 1031-3613
Statement of
P. D. Zalewski, X. Jian, L. L. Soon, W. G. Breed, R. F. Seamark, S. F. Lincoln, A. D. Ward and F. Z. Sun
Abstract: The Zn(II)-specific fluorophore Zinquin was used to determine the regional distribution of free or loosely-bound Zn(II) in mouse spermatozoa. Spermatozoa from the testes exhibited bright fluorescence over the entire head; those from the caput epididymides generally fluoresced more brightly in the post-acrosomal region; and spermatozoa from the caudae epididymides fluoresced less brightly, with foci of fluorescence over the sperm head which were lost after extraction with Triton X-100 and hence appeared to be membrane-associated. Treatment of cauda sperm with sodium dodecyl sulfate resulted in a bright uniform Zinquin fluorescence in the heads, similar to that observed in caput sperm, indicating that the two types of sperm have similar amounts of head Zn(II) but that the availability of Zn(II) for binding Zinquin is different. By contrast, the intensity of tail fluorescence was similar in spermatozoa from different regions of the male reproductive tract and was largely unaffected by Triton X-100 extraction, consistent with an intracellular location. Similar differences were observed between caput sperm and cauda sperm in the rat. It is concluded that visualization and measurement of free or loosely-bound Zn(II) in subcellular compartments of spermatozoa should facilitate investigation of the role of this metal in the development and function of spermatozoa and abnormalities that might accompany infertility and Zn(II) deficiency.
Keywords: Uterus
Edetic Acid
Tosyl Compounds
Chelating Agents
Fluorescent Dyes
Microscopy, Fluorescence
Rights: © CSIRO 1996
DOI: 10.1071/RD9961097
Appears in Collections:Anatomical Sciences publications
Aurora harvest 5
Environment Institute publications

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