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Type: Journal article
Title: Neutralizing monoclonal antibodies to different clades of Influenza A H5N1 viruses
Author: Oh, S.
Selleck, P.
Temperton, N.
Chan, P.
Capecchi, B.
Manavis, J.
Higgins, G.
Burrell, C.
Kok, T.
Citation: Journal of Virological Methods, 2009; 157(2):161-167
Publisher: Elsevier Science BV
Issue Date: 2009
ISSN: 0166-0934
Statement of
Sawyin Oh, Paul Selleck, Nigel J. Temperton, Paul K.S. Chan, Barbara Capecchi, Jim Manavis, Geoff Higgins, Christopher J. Burrell and Tuckweng Kok
Abstract: Four IgG(1kappa) monoclonal antibodies (mAbs) against Influenza A/Chicken/Vietnam/8/2004 (H5N1) virus are described. Three of these showed neutralizing activities against H5N1 strains from clades 1, 2 and 3 using a retroviral pseudotype or live virus microneutralization assay. In the pseudotype assay, the IC(90) neutralizing titre range was >1600-51,200, and with the microneutralization was 80> or =10,240. MAb 1C1 showed strong neutralizing activities in both assays. All four mAbs reacted specifically to the immunogen by immunohistochemical staining and to A/Hong Kong/483/1997 (H5N1) and A/Thailand/1(KAN-1)/2004 (H5N1)-infected MDCK cells by immunofluorescence. ELISA titrations of the mAbs showed specificity for H5N1 haemagglutinin (HA) and no cross-reactivity to 15 other Influenza A subtypes. Only mAbs 1C1 and the non-neutralizing 1F7 reacted with HA(1), the cleaved subunit of HA, by Western blot. These results suggest that the mAbs recognize distinct or overlapping epitopes and will be useful reagents for construction of specific rapid point-of-care assays or for therapeutic use.
Keywords: Animals; Chickens; Immunoglobulin G; Hemagglutinins, Viral; Antibodies, Monoclonal; Antibodies, Viral; Neutralization Tests; Inhibitory Concentration 50; Cross Reactions; Influenza A Virus, H5N1 Subtype
Description: Crown copyright © 2008 Published by Elsevier B.V.
RMID: 0020090643
DOI: 10.1016/j.jviromet.2008.12.016
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Appears in Collections:Medicine publications

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