Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/54775
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Cytochalasin B and trichostatin A treatment postactivation improves In Vitro development of porcine somatic cell nuclear transfer embryos |
Author: | Beebe, L. McIlfatrick, S. Nottle, M. |
Citation: | Cellular Reprogramming, 2009; 11(4):477-482 |
Publisher: | Mary Ann Liebert, Inc. Publishers |
Issue Date: | 2009 |
ISSN: | 1536-2302 1557-7457 |
Statement of Responsibility: | Luke F.S. Beebe, Stephen J. McIlfatrick, and Mark B. Nottle |
Abstract: | Somatic cell nuclear transfer (SCNT) is a useful technique for the production of transgenic pigs that can be used for biomedical research. However, the efficiency of SCNT in pigs is low. In this study, we examined the effect of two postactivation treatments, cytochalasin B (CB) and trichostatin A (TSA), on the in vitro development of porcine SCNT embryos. Treating porcine parthenotes with 7.5μg/mL CB for 3h after electrical activation was effective in preventing the extrusion of the second polar body in 65% of the oocytes compared to 17% in the control group. Treating SCNT embryos with CB for 3h after electrical activation significantly increased the average blastocyst cell number compared to the control group (CB treatment 51, Control 34, p<0.05). Treatment of porcine SCNT embryos with CB for 3h and 50nM TSA for 24h after electrical activation resulted in a threefold increase in blastocyst rate (CB+TSA 64%, CB 20%, p<0.05) and an increase in the average blastocyst cell number (CB+TSA 63, CB 46, p<0.05), compared to CB treatment alone. These results show that treatment with TSA and CB significantly improves the in vitro morphological development and quality of porcine SCNT embryos. |
Keywords: | Oocytes Blastocyst Animals Swine Hydroxamic Acids Cytochalasin B Protein Synthesis Inhibitors Cell Proliferation Pregnancy Parthenogenesis Drug Synergism Female Nuclear Transfer Techniques Embryo, Mammalian |
DOI: | 10.1089/clo.2009.0029 |
Published version: | http://dx.doi.org/10.1089/clo.2009.0029 |
Appears in Collections: | Aurora harvest 5 Obstetrics and Gynaecology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.