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|Title:||Apoptosis in liver damage produced by tunicamycin|
|Citation:||Australian Veterinary Journal, 2004; 82(1-2):87-90|
|Publisher:||Australian Veterinary Assn|
|Abstract:||OBJECTIVE:To determine whether apoptosis contributes to hepatocyte loss in tunicamycin poisoning. DESIGN:Groups of four guinea pigs were given 400 microg/kg of tunicamycin subcutaneously and killed at 24 h intervals up to 72 h post-injection. Livers were examined by routine histological methods and ancillary techniques (TUNEL staining, endonuclease activation, caspase activity, and electron microscopy) to ascertain whether any hepatocyte injury was apoptotic. RESULTS:Many hepatocytes exposed to tunicamycin showed evidence of apoptosis in the form of nuclear karyorrhexis with chromatin margination and crescent formation, TUNEL-positivity, DNA laddering, elevated caspase activity and apoptotic body formation. CONCLUSION:Tunicamycin caused the death of many hepatocytes in the livers of guinea pigs by apoptosis.|
|Keywords:||Liver; Animals; Animals, Domestic; Guinea Pigs; Plant Poisoning; Tunicamycin; Injections, Subcutaneous; In Situ Nick-End Labeling; Apoptosis; Animal Feed|
|Appears in Collections:||Pathology publications|
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