Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/5666
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Type: Journal article
Title: Differentiation of human bone-derived cells grown on GRGDSP-peptide bound titanium surfaces
Author: Zreiqat, H.
Akin, F.
Howlett, C.
Markovic, B.
Haynes, D.
Lateef, S.
Hanley, L.
Citation: Journal of Biomedical Materials Research. Part B: Applied Biomaterials, 2003; 64A(1):105-113
Publisher: John Wiley & Sons Inc
Issue Date: 2003
ISSN: 1552-4973
0021-9304
Statement of
Responsibility: 
H. Zreiqat, F. Ahu Akin, C. R. Howlett, B. Markovic, D. Haynes, S. Lateef, L. Hanley
Abstract: Various surface modifications have been applied to titanium alloy (Ti-6Al-4V) implants, in an attempt to enhance osseointegration; crucial for ideal prosthetic fixation. Despite the numerous studies demonstrating that peptide-modified surfaces influence in vitro cellular behavior, there is relatively little data reporting their effects on bone remodeling. The objective of this article was to examine the effects of chemically modifying Ti-6Al-4V surfaces with a common RGD sequence, a 15-residue peptide containing GRGDSP (glycine-arginine-glycine-aspartate-serine-proline), on the modulation of bone remodeling. The expression of proteins known to be associated with osseous matrix and bone resorption were studied during the growth of human bone-derived cells (HBDC) on these peptide-modified surfaces. HBDC grown for 7 days on RGD surfaces displayed significantly increased levels of osteocalcin, and pro-collagen Ialpha1 mRNAs, compared with the production by HBDC grown on the native Ti-6Al-4V. A pattern that was also reflected at the protein levels for osteocalcin, type I collagen, and bone sialoprotein. Moreover, HBDC grown for 7 and 14 days on RGD-modified Ti-6Al-4V expressed significantly higher level of osteoclast differentiation factors and lower levels of osteoprotegerin and IL-6 proteins compared with other surfaces tested. These results suggest that different chemical treatments of implant material (Ti-6Al-4V) surface result in differential bone responses, not only their ability to form bone but also to stimulate osteoclastic formation.
Keywords: peptides; osteoblasts; titanium; bone remodeling; osteoclasts
Description: Article first published online: 22 NOV 2002
Rights: © 2002 Wiley Periodicals, Inc.
RMID: 0020030678
DOI: 10.1002/jbm.a.10376
Appears in Collections:Pathology publications

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