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https://hdl.handle.net/2440/56680
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Type: | Journal article |
Title: | Low dose metal particles can induce monocyte/macrophage survival |
Author: | Lacey, D. De Kok, B. Clanchy, F. Bailey, M. Speed, K. Haynes, D. Graves, S. Hamilton, J. |
Citation: | Journal of Orthopaedic Research, 2009; 27(11):1481-1486 |
Publisher: | Elsevier Sci Ltd |
Issue Date: | 2009 |
ISSN: | 0736-0266 1554-527X |
Statement of Responsibility: | Derek C. Lacey, Bernard De Kok, Felix I. Clanchy, Mark J. Bailey, Kathy Speed, David Haynes, Stephen E. Graves, John A. Hamilton |
Abstract: | Aseptic loosening results in pain, loss of function, and ultimately prosthetic joint failure and revision surgery. The generation of wear particles from the prosthesis is a major factor in local osteolysis. We investigated the effects of such wear particles on the survival of monocytes and macrophages, populations implicated in wear particle-driven pathology. Particles from titanium aluminum vanadium (TiAlV) and cobalt chromium (CoCr) alloys were generated in-house and were equivalent in size (0.5-3 microm) to those seen in patients. Human CD14(+) monocytes and murine bone marrow-derived macrophages (BMM) were treated with TiAlV and CoCr particles in vitro, and cell survival was assayed. Both particles increased monocyte and macrophage survival in a dose-dependent manner, with an optimal concentration of around 10(7) particles/mL. Conditioned media from particle-treated BMM also increased macrophage survival. Studies with antibody blockade and gene-deficient mice suggest that particle-induced BMM survival is independent of endogenous CSF-1 (M-CSF), GM-CSF, and TNFalpha. These data indicate that wear particles can promote monocyte/macrophage survival in vitro possibly via an endogenous mediator. If this phenomenon occurs in vivo, it could mean that increased numbers of macrophages (and osteoclasts) would be found at a site of joint implant failure, which could contribute to the local inflammatory reaction and osteolysis. |
Keywords: | macrophages monocytes wear particles survival |
Description: | Copyright © 2009 Orthopaedic Research Society |
DOI: | 10.1002/jor.20914 |
Grant ID: | NHMRC |
Published version: | http://dx.doi.org/10.1002/jor.20914 |
Appears in Collections: | Aurora harvest Pathology publications |
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