Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/56682
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Type: Journal article
Title: Biological responses of human osteoblasts and osteoclasts to flame-sprayed coatings of hydroxyapatite and fluorapatite blends
Author: Bhadang, K.
Holding, C.
Thissen, H.
McLean, K.
Forsythe, J.
Haynes, D.
Citation: Acta Biomaterialia, 2010; 6(4):1575-1583
Publisher: Elsevier BV
Issue Date: 2010
ISSN: 1742-7061
1878-7568
Statement of
Responsibility: 
K.A. Bhadang, C.A. Holding, H. Thissen, K.M. McLean, J.S. Forsythe, D.R. Haynes
Abstract: The aim of this study was to determine how the activities of human osteoblastic cells and osteoclasts respond to substrates of thermal-sprayed mechanical blends of hydroxyapatite and fluorapatite with a view of determining an optimal blend ratio for osseointegration. Human osteoblastic cells and osteoclasts were grown on titanium alloy discs coated with blends of hydroxyapatite and fluorapatite, with concentrations ranging from 0 to 100% fluorapatite. Human osteoblastic cells attached in greater numbers and proliferated at a greater rate on blends containing 40% fluorapatite. Human osteoblastic cells grown on blends containing 40% fluorapatite for 7 days also expressed the highest levels of mRNA for several proteins involved with regulating bone metabolism (osteoprotegerin and receptor activator nuclear factor kappa B ligand), and bone formation (osteopontin, osteonectin and bone sialoprotein 1). Osteoclasts resorbed the dentine but poorly resorbed the hydroxyapatite-fluorapatite blends, particularly at high levels of fluorapatite. This in vitro study demonstrates that thermal-sprayed hydroxyapatitecoatings containing 40% fluorapatite may promote optimal bone growth and improve osseointegration of implants.
Keywords: Osteoblast; Osteoclast; Hydroxyapatite; Fluoride; Fluorapatite
Description: Copyright © 2009 Acta Materialia Inc. All rights reserved.
RMID: 0020094009
DOI: 10.1016/j.actbio.2009.10.029
Appears in Collections:Pathology publications

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