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|Title:||DNA from Buccal Swabs Suitable for High-Throughput SNP Multiplex Analysis|
|Citation:||Journal of Biomolecular Techniques, 2009; 20(5):232-235|
|Publisher:||The Association of Biomolecular Resource Facilities|
|Gai L. McMichael, Catherine S. Gibson, Michael E. O’Callaghan, Paul N. Goldwater, Gustaaf A. Dekker, Eric A. Haan, Alastair H. MacLennan, and for the South Australian Cerebral Palsy Research Group|
|Abstract:||We sought a convenient and reliable method for collection of genetic material that is inexpensive and noninvasive and suitable for self-collection and mailing and a compatible, commercial DNA extraction protocol to meet quantitative and qualitative requirements for high-throughput single nucleotide polymorphism (SNP) multiplex analysis on an automated platform. Buccal swabs were collected from 34 individuals as part of a pilot study to test commercially available buccal swabs and DNA extraction kits. DNA was quantified on a spectrofluorometer with Picogreen dsDNA prior to testing the DNA integrity with predesigned SNP multiplex assays. Based on the pilot study results, the Catch-All swabs and Isohelix buccal DNA isolation kit were selected for our high-throughput application and extended to a further 1140 samples as part of a large cohort study. The average DNA yield in the pilot study (n=34) was 1.94 μg ± 0.54 with a 94% genotyping pass rate. For the high-throughput application (n=1140), the average DNA yield was 2.44 μg ± 1.74 with a ≥93% genotyping pass rate. The Catch-All buccal swabs are a convenient and cost-effective alternative to blood sampling. Combined with the Isohelix buccal DNA isolation kit, they provided DNA of sufficient quantity and quality for high-throughput SNP multiplex analysis.|
|Keywords:||single nucleotide polymorphism|
|Description:||Copyright © 2009 Association of Biomolecular Resource Facilities|
|Appears in Collections:||Obstetrics and Gynaecology publications|
Cerebral Palsy Research Group publications
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