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Type: Journal article
Title: Quantifying oxygen diffusion in paraffin oil used in oocyte and embryo culture
Author: Stokes, Y.
Citation: Molecular Reproduction and Development, 2009; 76(12):1178-1187
Publisher: Wiley-Liss
Issue Date: 2009
ISSN: 1040-452X
Statement of
Yvonne M. Stokes
Abstract: Oxygen diffusion through oil is important in the culture of oocytes and embryos. A diffusion coefficient two orders of magnitude smaller than that of oxygen in water has been thought possible, and this has led to concerns of anoxia in cultures. Using an assay for determining the oxygen consumption rate of embryos and oocytes, along with a mathematical model, it is here shown that the oxygen diffusion rate in paraffin oil at 37 degrees C is about two-thirds of that in water at the same temperature. Although not previously recognised for the assay in question, the geometry is such that anoxia does occur for a period of time in excess of 1 hr and, by the completion of the assay, 30-40% of the medium is anoxic. Hence the quantity of oxygen consumed is less than would be consumed in conditions of plentiful oxygen supply. Nevertheless, using a model with a concentration dependent oxygen consumption rate, the oxygen consumption rate can be estimated.
Keywords: Oocytes
Cells, Cultured
Culture Media
Cell Culture Techniques
Oxygen Consumption
Models, Biological
Embryo, Mammalian
Description: Copyright © 2009 Wiley-Liss, Inc., A Wiley Company
DOI: 10.1002/mrd.21089
Appears in Collections:Aurora harvest 5
Mathematical Sciences publications

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