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|Title:||Temporal characterisation of pro- and anti-apoptotic mechanisms following diffuse traumatic brain injury in rats|
|Citation:||Journal of Clinical Neuroscience, 2002; 9(5):565-572|
|Ibolja Cernak, Sarah M. Chapman, Gary P. Hamlin and Robert Vink|
|Abstract:||Few studies have characterised apoptosis in a brain injury model that causes a significant degree of diffuse axonal injury. Such characterisation is essential from a clinical viewpoint since diffuse axonal injury is a major component of human head injury. The present study therefore, examines the expression of active and proactive caspase-3, and the bax, bcl-2 and bcl-x members of the bcl-2 family, to characterise the temporal profile of apoptosis in a model of traumatic brain injury in rats that produces significant diffuse axonal injury. Pentobarbital anaesthetised male Sprague-Dawley rats were injured using the 2m impact-acceleration model of diffuse traumatic brain injury. After injury, diffuse trauma resulted in an increased bax expression followed by induction of caspase-3. The increase in caspase-3 was simultaneous with an increase in anti-apoptotic bcl-2 expression. Bcl-x levels were increased after induction of caspase-3 and the increased levels of bcl-x were sustained to the end of the 5-day observation period. Increased active caspase-3 expression was associated with the appearance of TUNEL positive cells. These cells were detected in different brain regions at different times, with some regions showing no apoptotic cells until 3 days after injury. No TUNEL positive cells were detected at 7 and 14 days after injury. DNA electrophoresis confirmed that DNA fragmentation was maximal at 3 days after injury. Increased active caspase-3 levels were also significantly correlated with increased bcl-2 levels (r=0.80; P<0.001) suggesting that the apoptotic cascade after diffuse traumatic brain injury is a carefully controlled cellular homeostatic response. Pharmacological manipulation of this balance may offer a therapeutic approach for preventing cell death and improving outcome after diffuse traumatic brain injury.|
|Keywords:||Neurons; Animals; Rats; Rats, Sprague-Dawley; Brain Injuries; Caspases; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-bcl-2; Immunoblotting; Electrophoresis, Agar Gel; Immunohistochemistry; In Situ Nick-End Labeling; Apoptosis; DNA Fragmentation; Male; bcl-2-Associated X Protein; Caspase 3|
|Rights:||Copyright © 2002 Elsevier Science Ltd. All rights reserved.|
|Appears in Collections:||Pathology publications|
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