Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Elastic fiber proteins in the glomerular mesangium in vivo and in cell culture|
|Citation:||Kidney International, 2000; 58(4):1588-1602|
|Publisher:||Blackwell Publishing Inc|
|R Bernd Sterzel ; Andrea Hartner ; Ursula Schlötzer-schrehardt ; Susanne Voit ; Birgit Hausknecht ; Roberto Doliana ; Alfonso Colombatti ; Mark A Gibson ; Paola Braghetta ; Giorgio M Bressan|
|Abstract:||Glomerular capillaries of the mammalian kidney are exposed to high intraluminal hydrostatic pressures and require elastic constraint to maintain size, shape, and integrity. Previous morphological and functional studies indicated that the extracellular matrices of glomeruli, that is, basement membrane and mesangial matrix, contribute to glomerular resilience and mechanical stability. Immunofluorescence microscopy findings demonstrated elastic fiber components to be located in the renal vasculature, including glomeruli. The aim of this study was to clarify the exact glomerular localization, composition, and cellular production of these proteins.We examined the renal distribution of the elastic fiber proteins fibrillin-1, emilin, microfibril-associated glycoproteins (MAGPs) 1 and 2, latent transforming growth factor-binding protein-1 (LTBP-1), and elastin using immunohistology and immunoelectron microscopy of human, rat, and mouse kidneys. In mesangial cell cultures, we also studied the expression and extracellular deposition of such proteins by use of Northern blotting and immunocytochemistry.Fibrillin-1, emilin, MAGPs 1 and 2, and LTBP-1 were present in glomeruli of mouse, rat, and human kidney, where they were located predominantly in the mesangial extracellular matrix underlying glomerular endothelium and basement membrane. Several of these proteins, as well as elastin, were also expressed in the renal vasculature. While elastin localized to the glomerular vascular pole in afferent and efferent arterioles extending to Bowman's capsule, it was not found in the glomerular capillary tuft. Cultured mesangial cells of rat, mouse, and human kidneys expressed mRNAs of fibrillin-1, emilin, MAGP-2, and elastin, and the respective proteins localized within and outside of mesangial cells, as shown by immunocytochemistry. mRNA expression of fibrillin-1, emilin, and elastin was strong in quiescent mesangial cells; their gene expression was further up-regulated by transforming growth factor-beta1, while it was transiently reduced when cells were exposed to mitogenic 10% fetal calf serum and platelet-derived growth factor.These findings demonstrate that specific elastic fiber proteins are produced and secreted by mesangial cells. This process is regulated by growth factors. Their abundance in the extracellular matrix of the mesangium is in keeping with the concept that elastic fiber proteins contribute to the mechanical stability and elastic strength of the glomerular capillary tuft.|
|Keywords:||Glomerular Mesangium; Microcirculation; Cells, Cultured; Extracellular Matrix; Epithelial Cells; Animals; Humans; Mice; Rats; Elastin; Microfilament Proteins; Platelet-Derived Growth Factor; Proto-Oncogene Proteins c-sis; Transforming Growth Factor beta; Intracellular Signaling Peptides and Proteins; Carrier Proteins; Contractile Proteins; Membrane Glycoproteins; Extracellular Matrix Proteins; RNA, Messenger; Anticoagulants; Microscopy, Immunoelectron; Fluorescent Antibody Technique; Gene Expression; Homeostasis; Elasticity; Hydrostatic Pressure; Male; Latent TGF-beta Binding Proteins; Fibrillin-1; Fibrillins|
|Appears in Collections:||Pathology publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.