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Type: Journal article
Title: Single copy nuclear DNA markers for the onychophoran Phallocephale tallagandensis
Author: Sands, C.
Lancaster, M.
Austin, J.
Sunnucks, P.
Citation: Conservation Genetics Resources, 2009; 1(1):17-19
Publisher: Springer Netherlands
Issue Date: 2009
ISSN: 1877-7252
Statement of
Chester J. Sands, Melanie L. Lancaster, Jeremy J. Austin, Paul Sunnucks
Abstract: For some species, particularly invertebrates, developing single copy nuclear markers is an expensive and time-consuming task that may result in few or no usable markers. This has proven true for Onychophora (velvet worms). Here we describe our PCR-based method of generating single copy nuclear markers in Onychophora—a phylum comprised of species generally regarded as rare and of high conservation value—for which suitable microsatellites have been difficult to obtain. We list 6 primer pairs, some of which amplify across genera, and demonstrate their utility in identifying strong population structure in the species Phallocephale tallagandensis.
Keywords: Marker development; Anonymous single copy nuclear markers; Population genetics; Microsatellite; Onychophora
Rights: © Springer Science+Business Media B.V. 2009
RMID: 0020096266
DOI: 10.1007/s12686-009-9004-0
Grant ID:
Appears in Collections:Earth and Environmental Sciences publications
Australian Centre for Ancient DNA publications
Environment Institute Leaders publications
Environment Institute publications

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