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https://hdl.handle.net/2440/61365
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Type: | Journal article |
Title: | Chronic maternal hypertension affects placental gene expression and differentiation in rabbits |
Author: | McArdle, A. Maduwegedera, D. Moritz, K. Flower, R. Denton, K. Roberts, C. |
Citation: | Journal of Hypertension, 2010; 28(5):959-968 |
Publisher: | Lippincott Williams & Wilkins |
Issue Date: | 2010 |
ISSN: | 0263-6352 1473-5598 |
Statement of Responsibility: | Adelle M. McArdle, Devaki Maduwegedera, Karen Moritz, Rebecca L. Flower, Kate M. Denton and Claire T. Roberts |
Abstract: | <h4>Background</h4>Previously, we have shown that adult offspring from hypertensive rabbits develop hypertension.<h4>Method</h4>We aimed to determine the effects of mild (+15 mmHg) and moderate (+25 mmHg) increases in maternal blood pressure and plasma renin activity on placental differentiation and expression of components of the renin-angiotensin system and 11[beta]-hydroxysteroid dehydrogenase type 2 mRNA in rabbits. Placentas were collected from normotensive (sham), mild (2-kidney-1-cellophane wrapped; 2K-1W) and moderate (2-kidney-2-cellophane wrapped; 2K-2W) hypertensive groups at gestational age of 14, 21 and 28 days. Placental gene expression was quantified by reverse transcriptase-PCR, and morphometry was assessed by videoimage analyses of placental sections.<h4>Results</h4>Fetal weight was similar between groups across gestation. In the 2K-1W group at gestational age day 14, fetal-to-placental weight ratio was increased (approximately 34%) as were volumes of fetal capillaries ([up arrow]56%) and maternal blood space at gestational age day 21 ([up arrow]55%) compared with sham (all P < 0.05). In the 2K-2W group, fetal-to-placental weight ratio was increased at gestational age day 21 (approximately 25%; P < 0.01) with an accompanying reduction in placental weight, and at gestational age day 28, volume density of fetal capillaries was increased (approximately 22%; P < 0.05). Placental renin mRNA was lower in both the 2K-1W (approximately 88%) and 2K-2W (approximately 98%) groups at gestational age day 28 (all P < 0.01). Placental 11[beta]-hydroxysteroid dehydrogenase type 2 mRNA was lower in the 2K-1W (approximately 36%) and 2K-2W (approximately 31%) groups at gestational age day 14 and greater (approximately 36%) in the 2K-2W group at gestational age day 21 (all P < 0.01). Associations between placental AT1R and AT2R mRNA and placental differentiation were disturbed by hypertension.<h4>Conclusion</h4>Mild and moderate maternal hypertension differentially alters placental structure and gene expression that may affect placental functional capacity and contribute to programming of hypertension in offspring. |
Keywords: | chronic hypertension placenta pregnancy programming rabbit renin-angiotensin system |
Rights: | © 2010 Lippincott Williams & Wilkins, Inc. |
DOI: | 10.1097/HJH.0b013e3283369f1e |
Published version: | http://dx.doi.org/10.1097/hjh.0b013e3283369f1e |
Appears in Collections: | Aurora harvest 5 Obstetrics and Gynaecology publications |
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